Prolamin composition and preparation method thereof
A technology of gliadin and composition, which is applied in the direction of protein food processing, protein food ingredients, dairy products, etc., can solve the problems of non-suitability for non-dairy cream, poor spreadability, etc., and achieve good instant solubility, high whipping ratio, and convenient storage Effect
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[0057] 14. A preparation method of gliadin composition, said method comprising the steps of:
[0058] (1) Dissolving 50-95 parts by weight of prolamin and optionally 0-28 parts by weight of polyhydroxy compound in 375-712 parts by weight of 70v / v% ethanol solution to obtain the first feed solution, wherein, The ethanol solution is at 45~55°C;
[0059] (2) Dissolving optionally 0-150 parts by weight of dextrin and optionally 0-28 parts by weight of polyhydroxy compound in 987-1874 parts of water to obtain a second feed liquid;
[0060] (3) Add 250 to 475 parts by weight of 40v / v% ethanol solution into the first feed liquid of step (1), and stir to dissolve the solute again to obtain a primary reverse phase precipitate, wherein the ethanol The solution is at 45-55°C;
[0061] (4) Add the primary reverse phase eluate obtained in step (3) to the second feed liquid obtained in step (2) and stir at a reaction temperature of 0-25°C; remove insoluble substances to obtain a secondary...
Embodiment 1
[0092] (1) Raw material dissolution: 50 parts by weight of zein (α-gliadin, β-gliadin and γ-gliadin account for 97wt%, 2wt% and 0% of gliadin respectively) are completely dissolved in In 375 parts by weight of 70v / v% ethanol solution at 50°C, the pH was adjusted to 6 to obtain the first feed solution.
[0093] (2) 25 parts by weight of dextrin were completely dissolved in 987 parts by weight of water to obtain a second feed liquid.
[0094] (3) Primary reverse phase precipitation: 250 parts by weight of 40v / v% ethanol (50°C) was added to the first feed liquid obtained in step (1), and stirred rapidly, and stirred and kept at 50°C for 30min, A reversed-phase precipitate was obtained.
[0095] (4) Secondary reverse-phase precipitation: Add the primary reverse-phase precipitation in step (3) to the second material liquid obtained in step (2) and stir for rapid dispersion. The reaction temperature is controlled at 15°C, and the pore size is 100 μm. After the filter cloth removes...
Embodiment 2
[0099] (1) Dissolving raw materials: 60 parts by weight of zein (alpha-gliadin, beta-gliadin and gamma-gliadin respectively account for 90wt%, 5wt% and 1wt% of the prolamin), 7 wt% Parts of glycerol and 3 parts by weight of polyethylene glycol were completely dissolved in 450 parts by weight of 70v / v% ethanol solution at 45°C, and the pH was adjusted to 2.8 to obtain the first feed solution.
[0100] (2) 18 parts by weight of chitosan were completely dissolved in 1184 parts by weight of water to obtain a second feed liquid.
[0101] (3) One-time reverse phase precipitation: 300 parts by weight of 40v / v% ethanol (45°C) was added to the first feed liquid obtained in step (1), and stirred rapidly, and kept at 45°C for 30min to obtain A reverse phase eluate.
[0102] (4) Secondary reverse phase precipitation: Add the primary reverse phase precipitation solution in step (3) to the second material liquid obtained in step (2) and stir to make rapid dispersion. The reaction temperature...
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