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Masson staining solution two-color method formula

A dyeing solution and formula technology, applied in the field of pathology, can solve the problems of narrow latitude of coloring conditions, insufficient brilliance, inability to dye muscle fibers and collagen fibers, etc., and achieve the effects of operator and environmental safety, easy promotion, and low volatility.

Inactive Publication Date: 2019-07-05
宁波同盛生物科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The traditional Masson staining solution uses Ponceau 2R and acid fuchsin as the main components, and has the following defects: 1. It can only stain muscle fibers alone, and cannot stain muscle fibers and collagen fibers at the same time; 2. Ponceau 2R and The coloring condition of acid fuchsin has a narrow tolerance, and fine differentiation steps are required after staining; 3. Using traditional Masson staining solution to observe the muscle fibers in tissue sections under a microscope, the color is not bright enough

Method used

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Embodiment Construction

[0013] In order to make the object, technical solution and advantages of the present invention clearer, the present invention is further described in detail.

[0014] A Masson staining solution two-color method formula, including the following components: chromotropic acid 2R, fast green, phosphotungstic acid, glacial acetic acid, water, and the various components by weight percentage are: chromotropic acid 2R: 0.5% to 0.8%, solid Green: 0.1% to 0.4%, glacial acetic acid: 0.5% to 1.5%, phosphotungstic acid: 0.5% to 1.0%, water: 98.5% to 99.5%, and the percentages by weight of the various ingredients are: color changing acid 2R: 0.6 %~0.7%, Fast Green: 0.18%~0.25%, Glacial Acetic Acid: 0.8%~1.2%, Phosphotungstic Acid: 0.75%~0.85%, Water: 98.5%~99.5%.

[0015] Compared with the prior art, the present invention has the advantages of:

[0016] 1. The operation process is simple and fast: the four steps of the traditional dyeing process are combined into one step, which significan...

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Abstract

The invention relates to a Masson staining solution two-color method formula, which comprises the following components: a chromotropic acid 2R, fast green, a phosphotungstic acid, a glacial acetic acid and water. The operation flow of the Masson staining solution two-color method formula is simple and quick, and four steps of a traditional staining process are combined into one step. The Masson staining solution two-color method formula significantly optimizes the operation process and is easy to popularize.

Description

technical field [0001] The invention relates to the field of pathological technology, in particular to a Masson staining liquid two-color method formula. Background technique [0002] At present, in the field of pathological technology, the traditional Masson staining solution formula and operation process are mostly used for the staining of collagen fibers in paraffin sections. The traditional Masson staining solution uses Ponceau 2R and acid fuchsin as the main components, and has the following defects: 1. It can only stain muscle fibers alone, and cannot stain muscle fibers and collagen fibers at the same time; 2. Ponceau 2R and The coloring conditions of acid fuchsin have a narrow tolerance, and fine differentiation steps are required after staining; 3. Using traditional Masson staining solution to observe the muscle fibers in tissue sections under a microscope, the color is not bright enough. Contents of the invention [0003] Regarding the present situation of the a...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 尤丽丽
Owner 宁波同盛生物科技有限公司
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