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Single-chain antibody and chimeric antigen receptor T cells targeting DR5, and preparation method and application thereof

A technology of chimeric antigen receptors and single-chain antibodies, which is applied in the field of medical biology to achieve strong affinity activity, promotion of amplification, high-efficiency and specific killing effects

Inactive Publication Date: 2019-07-02
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no preparation and research of chimeric antigen receptor T cells targeting DR5

Method used

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  • Single-chain antibody and chimeric antigen receptor T cells targeting DR5, and preparation method and application thereof
  • Single-chain antibody and chimeric antigen receptor T cells targeting DR5, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] A method for preparing chimeric antigen receptor T cells targeting DR5, comprising the following steps:

[0076] (1) Prepare the gene sequence of chimeric antigen receptor CAR-DR5 targeting DR5

[0077]The genes encoding the signal peptide, the single-chain antibody targeting DR5, the hinge region of CD8α, the transmembrane region of CD8, the signal region of CD28 and the signal region of CD3ζ were prepared respectively, and the genes encoding the signal peptide were shown in SEQ ID NO: 15. The coding gene of the single-chain antibody targeting DR5 is shown in SEQ ID NO: 2, the coding gene of the CD8α hinge region is shown in SEQ ID NO: 7, and the coding gene of the CD8 transmembrane region is shown in SEQ ID NO 9, the coding gene of the CD28 signal region is shown in SEQ ID NO: 11, and the coding gene of the CD3ζ signal region is shown in SEQ ID NO: 13.

[0078] The genes encoding the above signal peptide, single-chain antibody targeting DR5, CD8α hinge region, CD8 tr...

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PUM

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Abstract

The present invention provides a single-chain antibody targeting DR5. The single-chain antibody targeting the DR5 comprises an amino acid sequence shown as SEQ ID NO:1. The present invention also provides chimeric antigen receptor T cells comprising the single-chain antibody targeting the DR5. Chimeric antigen receptors targeting the DR5 can specifically target the DR5, promote T cell proliferation in patient body, efficiently and specifically kill tumor cells, better maintain cell viability and lethality, and also do not cause damages on normal cells. The present invention also provides a preparation method and an application of the chimeric antigen receptor T cells targeting the DR5.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a single-chain antibody targeting DR5, a chimeric antigen receptor T cell, and a preparation method and application thereof. Background technique [0002] Cancer has become the culprit that threatens human life, and the incidence rate is on the rise. From the perspective of inducing tumor cell apoptosis or overcoming the resistance of tumor cells to apoptosis, people have discovered tumor necrosis factor-related apoptosis-inducing ligand (TRAIL ), a member of the tumor necrosis factor superfamily capable of inducing apoptosis. Death receptor 5 (death receptor5; DR5) is a member of the tumor necrosis factor receptor superfamily (TNFRSF10B), and its cytoplasmic region contains a death domain, mainly distributed in tumor cells. TRAIL binds to DR5 on the cell surface and induces apoptosis. DR5 is widely expressed in various solid tumor cells such as liver cancer, while it is very wea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C12N15/13C07K19/00C12N15/62C12N15/867C12N5/10A61P35/00
CPCA61K35/17C07K14/7051C07K16/2878C07K2317/24C07K2317/622C07K2319/02C07K2319/03C07K2319/33C07K2319/74C12N5/0636C12N15/86C12N2510/00C12N2740/15043C12N2800/107
Inventor 张宏玲龙丽梅钟春颖
Owner SHENZHEN BINDEBIOTECH CO LTD
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