Single-chain antibody and chimeric antigen receptor T cells targeting DR5, and preparation method and application thereof
A technology of chimeric antigen receptor and single chain antibody, which is applied in the field of medical biology to achieve the effect of promoting amplification, strong affinity activity, high efficiency and specific killing
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[0075] A method for preparing chimeric antigen receptor T cells targeting DR5, comprising the following steps:
[0076] (1) Prepare the gene sequence of chimeric antigen receptor CAR-DR5 targeting DR5
[0077]The genes encoding the signal peptide, the single-chain antibody targeting DR5, the hinge region of CD8α, the transmembrane region of CD8, the signal region of CD28 and the signal region of CD3ζ were prepared respectively, and the genes encoding the signal peptide were shown in SEQ ID NO: 15. The coding gene of the single-chain antibody targeting DR5 is shown in SEQ ID NO: 2, the coding gene of the CD8α hinge region is shown in SEQ ID NO: 7, and the coding gene of the CD8 transmembrane region is shown in SEQ ID NO 9, the coding gene of the CD28 signal region is shown in SEQ ID NO: 11, and the coding gene of the CD3ζ signal region is shown in SEQ ID NO: 13.
[0078] The genes encoding the above signal peptide, single-chain antibody targeting DR5, CD8α hinge region, CD8 tr...
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