Device and method for separating and purifying anthocyanidin

Abstract

The invention discloses a device and a method for separating and purifying anthocyanidin. The device comprises a central distribution tank, and the feed inlet of the central distribution tank is respectively connected with an anthocyanin concentrate feeding tube, an eluent feeding tube and a pure water supply tube; a resin is arranged in a chromatography column, the top of the chromatography column is provided with an activation liquid inlet and a feed port, the bottom of the chromatography column is provided with a discharge main tube, and the feed port is communicated with the discharge portof the central distribution tank; the chromatography column is also provided with a resin replacement inlet and a resin replacement outlet; and the discharge man tube is respectively connected with an eluent storage tank, a byproduct storage tank, a high-purity liquid storage tank and a low-purity liquid storage tank, the discharge main tube is further provided with an activation liquid outlet joint and a sewage discharge outlet joint, the discharge main tube is provided with a liquid chromatography detector, and the activation liquid outlet joint is connected with the activation liquid storage tank. The separation and purification device can keep a high separation and purification efficiency for a long time, and is suitable for the automatic extraction process of anthocyanin.

Description

Technical field [0001] The invention relates to a separation and purification device, in particular to a separation and purification device for anthocyanins. Background technique [0002] There are many types of natural anthocyanidins, which are a kind of substances widely found in plants in nature. Anthocyanidins are water-soluble natural pigments. Most of the main coloring substances in fruits, vegetables and flowers are related to it. Under different pH conditions of plant cell vacuoles, anthocyanins make petals show colorful colors. There are more than 20 kinds of anthocyanins, of which 6 are important in foods, namely geranium pigment, cornflower pigment, delphinium pigment, peony pigment, morning glory pigment and malva pigment. The natural anthocyanins exist in the form of glycosides, called anthocyanins, and there are few free anthocyanins. Anthocyanins are mainly used in food coloring, but also in dyes, medicine, cosmetics, etc. The anthocyanins contained in blueberr...

AI Technical Summary

Problems solved by technology

The blueberry extract is concentrated to form anthocyanin concentrate, which also contains some other impurities, so it needs to be separated, purified and separated, and one of the methods of separation, purification and separation is to use separation The purification device separates, purifies and refines, but the effect of the current separation and purification device is not obvious. Because the separation and purification device uses resin as the adsorption carrier of anthocyanin, the resin will be inactivated after several times of use, and the effect of separation and purification will be reduced. Reduced, so it is not suitable for the continuous extraction process of anthocyanins

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