Serum-free, animal-derived-component-free culture medium with definite components and application thereof
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An animal-derived, serum-free technology, applied in the field of cell culture, can solve the problems of low cell expansion efficiency, affecting safety and stability, and unclear components, achieving good culture effect and alleviating the effect of poor repeatability.
Active Publication Date: 2019-06-28
REGEN-GEEK (SHENZHEN) MEDICAL TECH CO LTD
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Although the traditional culture medium added with serum and other animal-derived components can realize the in vitro culture of human cells, there are substances that damage cell growth and reproduction, such as complement, immunoglobulin and some growth inhibitory factors, and the components are not clear; traditional culture The reproducibility of the base for cell culture is also poor, which affects the safety and stability of clinical (human) application; and the serum activity of different animals and different batches is quite different, which affects the stability of the culture effect; at the sa
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[0166] As a preferred embodiment of the present invention, the serum-free, animal-derived component-free, and defined medium includes basal medium, and cytokines, hormones, and transferrin added to the basal medium , albumin, fetuin, putrescine, serotonin, fibronectin, amino acids, lipids, trace elements, vitamins, surfactants, antioxidant protective agents, pH buffers and catechins.
[0167] In a second aspect, the present invention provides a serum-free, animal-derived component-free, defined medium for the growth of mesenchymal stem cells, fibroblasts, chondrocytes, neural stem cells, hematopoietic cells, and other mesoderm and ectoderm sources. application in cell culture.
[0168] In the present invention, a serum-free, animal-derived component-free, and defined medium is obtained by adding substances such as cytokines, hormones, transferrin, albumin, fetuin, putrescine, and serotonin to the basal medium . The substances of each component of the medium are clear, and th...
Embodiment 1
[0171] This example provides a serum-free, animal-derived component-free medium (RZXF) with defined components, the contents of which are shown in Table 1.
[0172] Table 1 Serum-free, animal-derived component-free, and defined medium provided by Example 1 of the present invention
[0173]
[0174]
[0175]
[0176] Note: The concentration of each substance in the table is the concentration of additional addition, not including the concentration of the substance already in the basal medium.
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Abstract
The invention provides a serum-free, animal-derived-component-free culture medium with definite components and application thereof, and relates to the technical field of cell culture. The serum-free and animal-derived-component-free culture medium with definite components comprises a basic culture medium and cytokines, hormones, transferrin, albumin, fetal globulin, putrescine and serotonin whichare added into the basic culture medium. According to the serum-free, animal-derived-component-free culture medium with definite components, substances such as cytokines, hormones and the like are added into a basic culture medium to replace serum, so that the culture of human cells is realized. The components of the culture medium are definite, no serum and animal-derived-component components areadded, so that the technical problems of poor repeatability of the traditional serum-containing culture medium for cell culture, low cell activity and low cell amplification efficiency of other serum-free culture mediums can be mitigated.
Description
technical field [0001] The invention relates to the technical field of cell culture, in particular to a serum-free, animal-source component-free medium with defined components and an application thereof. Background technique [0002] Cell culture technology is an important and commonly used technology in cell biology research methods. By simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro, the cells can survive, grow, reproduce and maintain the main structure and function. Obtain a large number of cells to study cell signal transduction, anabolism, growth and proliferation, etc. Of all cell cultures in vitro, the most difficult is human cell culture. [0003] The nutrients required for in vitro cell culture are basically the same as those in the body. For example, sugars, amino acids, inorganic salts, growth-promoting factors, trace elements, etc. are required. The above-mentioned substances required by ...
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