Intracellular fluorescence labeling method of food-derived probiotics and in-vivo application thereof

Abstract

The invention relates to an intracellular fluorescence labeling method of food-derived probiotics. The method comprises the following steps that a, a long-afterglow nano-luminescent material is modified by using a DNA; b, a probiotic competence is prepared; c, a DNA coated nano fluorescent probe is electrically transferred to the probiotics; d, the nano fluorescent probe is orally taken into a mouse body for imaging. The method has the advantages that the method for using the nano-luminescent material for intracellular fluorescence labeling is achieved for the first time, complex surface functionalization and antibody modification of a fluorescent material are not needed, the steps are simple and convenient, the cost is low, and compared with surface marking, the method has better stability and marking reproducibility. According to a near-infrared fluorescence labeling probiotic body in-vivo biological imaging technology developed through the method, the long-afterglow nano-luminescentproperty inside thalli can be used, metabolic behaviors and distribution conditions of probiotic bodies in a biological body can be monitored losslessly in situ in real time, and an innovative research means is provided for functional sites and nutriology of the food-derived probiotics.

Description

technical field [0001] The invention belongs to the research field of food-derived probiotics, and relates to a method for intracellular fluorescent labeling of food-derived probiotics and its application in vivo. Background technique [0002] The Food and Agriculture Organization of the United Nations and the World Health Organization point out that probiotics are a type of living microorganisms that, when ingested in a certain amount, have beneficial effects on the health of the host. Studies have shown that probiotics can resist the invasion of pathogenic bacteria, inhibit the reproduction of pathogenic bacteria, improve the barrier function of intestinal epithelial cells, maintain the homeostasis of the gastrointestinal tract, and rebuild the stability of intestinal flora. Probiotics play a beneficial role mainly through the production of dominant flora, biological oxygen capture and biological antagonism, and through the production of beneficial substances and antibacte...

AI Technical Summary

Problems solved by technology

However, these two methods still have problems such as low sensitivity and short emission wavelength, which have certain limitations when applied to in vivo imaging.

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