Method for detecting biological radiation damage by using SERS substrate

A technology for biological radiation and radiation damage, which is applied in the fields of radiation therapy and biological analysis and detection, and achieves the effects of improving the signal-to-noise ratio, broad application prospects and simple preparation process.

Inactive Publication Date: 2019-06-18
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there have been some reports on this research, there is currently no standard and general method

Method used

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  • Method for detecting biological radiation damage by using SERS substrate
  • Method for detecting biological radiation damage by using SERS substrate
  • Method for detecting biological radiation damage by using SERS substrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation method of SERS substrate, its specific steps are as follows:

[0046] (1) Preparation of anodic aluminum oxide (AAO) templates by a step-by-step method:

[0047] a. For a high-purity (mass fraction of 99.99%) metal aluminum film, use an oxalic acid solution with a concentration of 0.3mol / L, oxidize for 7 hours at a temperature of 0°C, and a voltage of 45V;

[0048] b. Put the sample obtained in step a into a mixture of phosphoric acid:chromic acid (volume ratio 6:1.8), at a temperature of 50°C, and expand the holes for 10 hours;

[0049] c. Place the sample obtained in step b into a 5% phosphoric acid solution at a temperature of 45°C and an initial voltage of 45V, decreasing by 2V every 3 minutes to 31V; when the voltage drops to 31V, oxidize for 6h;

[0050] d. The anodized aluminum template obtained in step c is soaked in ultrapure water and cleaned for 2 hours, and placed in the air to dry naturally.

[0051] (2) Au sputtering of anodized aluminum ...

Embodiment 2

[0057] The preparation method of SERS substrate, its specific steps are as follows:

[0058] (1) Preparation of anodic aluminum oxide (AAO) templates by a step-by-step method:

[0059] a. For a high-purity (mass fraction of 99.99%) metal aluminum film, use an oxalic acid solution with a concentration of 0.1mol / L, oxidize for 8 hours at a temperature of 0°C and a voltage of 40V;

[0060] b. Put the sample obtained in step a into a mixture of phosphoric acid:chromic acid (volume ratio 6:1.8), at a temperature of 40°C, and expand the holes for 9 hours;

[0061] c. Place the sample obtained in step b into a 5% phosphoric acid solution at a temperature of 40°C and an initial voltage of 45V, decreasing by 2V every 3 minutes until it reaches 31V; when the voltage drops to 31V, oxidize for 6h;

[0062] d. The anodized aluminum template obtained in step c is soaked in ultrapure water and cleaned for 1 hour, and then dried naturally in the air.

[0063] (2) Au sputtering of the anodiz...

Embodiment 3

[0068] The preparation method of SERS substrate, its specific steps are as follows:

[0069] (1) Preparation of anodic aluminum oxide (AAO) templates by a step-by-step method:

[0070] a. For a high-purity (mass fraction of 99.99%) metal aluminum film, use an oxalic acid solution with a concentration of 0.4mol / L at a temperature of 0°C and a voltage of 55V for 8 hours of oxidation;

[0071] b. Put the sample obtained in step a into a mixture of phosphoric acid:chromic acid (volume ratio 6:1.8), at a temperature of 60°C, and expand the holes for 9 hours;

[0072] c. Place the sample obtained in step b into a 5% phosphoric acid solution at a temperature of 50°C and an initial voltage of 45V, decreasing by 2V every 3 minutes to 31V; when the voltage is reduced to 31V, oxidize for 6h;

[0073] d. The anodized aluminum template obtained in step c is soaked in ultrapure water and cleaned for 1 hour, and then dried naturally in the air.

[0074] (2) Au sputtering of the anodized al...

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Abstract

The invention discloses a method for detecting a biological radiation damage by using a SERS substrate and relates to the radiation radiotherapy and bioanalysis detection field. The method comprises the following steps of (1) determining a spectral signal of a biomarker related to the radiation damage through using a Raman spectrometer; and (2) dropping a sample onto the SERS substrate, then usingthe Raman spectrometer to carry out SERS detection, and comparing a detection result with the spectral signal to determine whether the sample is damaged by radiation. The method has advantages that aSERS spectrum is discovered and proposed to be used to measure a radiation damage signal; a manufacturing method of the SERS substrate is simple and rapid and has good stability; and the method can be used to carry out highly-sensitive detection and analysis on the radiation biomarker.

Description

technical field [0001] The invention relates to the fields of radiation therapy and biological analysis and detection, in particular to a method for detecting biological radiation damage using a SERS substrate. Background technique [0002] Radiation damage refers to acute, delayed or chronic tissue damage caused by ionizing radiation, which can be divided into acute, chronic damage and long-term effects, among which acute damage is more common in nuclear radiation accidents. Radiation injury poses a serious threat to human health and requires timely and effective diagnosis and treatment. In addition, radiation in the process of radiotherapy also causes harm to the human body. [0003] At present, the analysis, detection and evaluation technology of radiation injury mainly depends on the slow time effect of radiation symptoms, and there are still some difficulties in the timely detection of accidental radiation injury personnel. For example, moderate to high damage to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N33/50
Inventor 黄青穆罕默德邵长胜
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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