Method for extracting and obtaining bromelain from pineapple peel residues
A technology of bromelain and pineapple peel residues, applied in the direction of sterilization methods, methods of supporting/immobilizing microorganisms, biochemical equipment and methods, etc., can solve waste of raw materials, can not effectively improve enzyme purity and enzyme activity, pineapple peel Solve the problems of low utilization rate of slag and enzyme yield, and achieve the effect of improving utilization rate, improving utilization rate and enzyme yield, and high juice extraction rate
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Embodiment 1
[0033] A method for extracting and obtaining bromelain from pineapple pomace, comprising the following steps:
[0034] Step 1. Wash the pineapple skin residue, first put it into a crushing and filtering device for multi-stage crushing, then add 0.1% ascorbic acid, and add 1Kg of distilled water, and finally obtain the filtrate by squeezing and filtering;
[0035] Step 2, add 0.05% calcium propionate to the above filtrate for anticorrosion, stir and centrifuge the filtrate at a high speed for 15 minutes at a stirring speed of 5000r / min, take the supernatant, and add pre-dissolved tannin solution to the supernatant, while Stir while adding, so that the final concentration of tannin is 0.2%. After adding tannin, let it stand still, and after the formed tannin-enzyme complex is precipitated, carefully remove the supernatant by siphon method to obtain the lower compound The concentrated solution of the compound; the concentrated solution of the lower complex is separated and washed...
Embodiment 2
[0041] A method for extracting and obtaining bromelain from pineapple pomace, comprising the following steps:
[0042] Step 1. Wash the pineapple skin residue, first put it into a crushing and filtering device for multi-stage crushing, then add 0.05% ascorbic acid, and add 0.5Kg distilled water, and finally obtain the filtrate by squeezing and filtering;
[0043] Step 2, add 0.03% calcium propionate to the above filtrate for anticorrosion, stir and centrifuge the filtrate at a high speed for 10 minutes at a stirring speed of 4000r / min, take the supernatant, and add pre-dissolved tannin solution to the supernatant, while Stir while adding, so that the final concentration of tannin is 0.1%. After adding tannin, let it stand still, and after the formed tannin-enzyme complex is precipitated, carefully remove the supernatant by siphon method to obtain the lower layer compound The concentrated solution of the compound; the concentrated solution of the lower complex is separated and ...
Embodiment 3
[0048] Step 1. Wash the pineapple skin residue, first put it into a crushing and filtering device for multi-stage crushing, then add 0.1% ascorbic acid, and add 0.5Kg distilled water, and finally obtain the filtrate by squeezing and filtering;
[0049] Step 2, add 0.05% calcium propionate to the above filtrate for anticorrosion, stir and centrifuge the filtrate at a high speed for 10 minutes at a stirring speed of 5000r / min, take the supernatant, and add pre-dissolved tannin solution to the supernatant, while Stir while adding, so that the final concentration of tannin is 0.1%. After adding tannin, let it stand still, and after the formed tannin-enzyme complex is precipitated, carefully remove the supernatant by siphon method to obtain the lower layer compound The concentrated solution of the compound; the concentrated solution of the lower complex is separated and washed to prepare an enzyme paste, and the enzyme paste is placed in a low-temperature freezer below 12°C for 20 h...
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