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A kind of method for cultivating NK cells in vitro

A culture method, NK cell technology, applied in animal cells, vertebrate cells, blood/immune system cells, etc.

Active Publication Date: 2020-09-18
山东翰康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is known that chebulic acid has various pharmacological activities, but there is no report that chebulic acid promotes the proliferation of NK cells in vitro and improves its killing activity

Method used

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  • A kind of method for cultivating NK cells in vitro
  • A kind of method for cultivating NK cells in vitro
  • A kind of method for cultivating NK cells in vitro

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Experimental program
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Embodiment Construction

[0024] The following describes the substantive content of the present invention in conjunction with the accompanying drawings and embodiments, but does not limit the protection scope of the present invention.

[0025] 1. Experimental materials

[0026] Lymphocyte separation medium was purchased from Beijing Suolaibao Technology Co., Ltd.; rhIL-2 was purchased from Xiamen Tebao Bioengineering Co., Ltd.; human AB plasma was purchased from Shanghai Huiying Biotechnology Co., Ltd.; SCGM serum-free medium was purchased from Shanghai Micro Branch Biotechnology Co., Ltd.; FITC-Anti-CD56, PE-Anti-CD3, APC-Anti-CD107a were purchased from BD Company in the United States; PBS solution was homemade; chebulic acid was purchased from Chengdu Prefa Technology, with a purity of ≥95%; CCK -8 reagent was purchased from Beyontien; MKN-45 human gastric cancer cells were purchased from Shanghai Kanglang Biotechnology Co., Ltd.

[0027] 2. Experimental method

[0028] 1. NK cell culture and pheno...

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Abstract

The invention discloses a method for in-vitro culture of NK cells. It is found that chebulinic acid can promote in-vitro proliferation of the NK cells and can remarkably improve the broad-spectrum killing activity of the NK cells and the killability of cancer cells, that is, the expression of the killing activity biomarker CD107a is significantly improved and the killability of MKN-45 human gastric carcinoma cells is remarkable, and when chebulinic acid intervenes with culture, the phenotype and the content of the NK cells cannot be changed. Therefore, the NK cells can be cultured in vitro byusing chebulinic acid, NK cell proliferation is promoted, the killability of the NK cells is improved, and the chebulinic acid can be added into a culture medium to be developed into a culture mediumspecial for in-vitro culture of the NK cells.

Description

technical field [0001] The invention belongs to the biological field and relates to immune cell culture, in particular to a method for culturing NK cells in vitro. Background technique [0002] Natural killer cells (NK) are one of the first innate immune defense members in the human body, and they are also the strongest and most effective immune lymphocytes against cancer cells or viruses, and other anti-cancer immune cells such as toxic T cells Compared with (CTL) or dendritic cells (DC), NK cells have a broad spectrum, are not limited by histocompatibility antigens, and can directly attack cancer cells without the initiation of sensitization, so they are called natural killer cells. Killer cells. Clinical experiments have shown that after the immune cells of tumor patients are obtained, stimulated in vitro, expanded and cultured, and then infused back into the patient's body, it can directly kill tumor cells, restore or enhance the patient's immune function and inhibit tu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783
Inventor 石学兵杨浩
Owner 山东翰康生物科技有限公司
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