Establishment method of rat model of endometriosis with kidney deficiency and blood stasis syndrome

A technology for kidney deficiency, blood stasis, and endometrium, which is applied in the field of life sciences, can solve the problem of difficult control of experimental animal models of TCM syndromes, lack of accurate and effective ways to identify syndrome characteristics, TCM clinical diagnosis, efficacy evaluation and new drug development Difficulties in international communication and other issues

Inactive Publication Date: 2019-06-14
SHANXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0003] However, the main problems at present are: it is difficult to control the experimental animal models of TCM syndromes, or directly use the method of disease modeling, and lack of accurate and effective ways to identify the characteristics of syndromes. Difficulties in communication between

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  • Establishment method of rat model of endometriosis with kidney deficiency and blood stasis syndrome
  • Establishment method of rat model of endometriosis with kidney deficiency and blood stasis syndrome
  • Establishment method of rat model of endometriosis with kidney deficiency and blood stasis syndrome

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[0037] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0038] The establishment method of rat model of endometriosis with kidney deficiency and blood stasis syndrome, prepare 3-month-old female SD rats, weighing about 200g, EMs rats with kidney deficiency and blood stasis syndrome, conduct vaginal cell smear examination every day, and select estrous cycle 4 ~5 days are qualified animals, first establish EMs SD rat model of kidney deficiency and blood stasis syndrome, including the following steps:

[0039] Step 1:...

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Abstract

The invention discloses an establishment method of rat model of endometriosis with kidney deficiency and blood stasis syndrome. The method comprises the following steps: firstly, a SD rat model of EMskidney deficiency and blood stasis syndrome is established, including the following processes: after one week of normal feeding, the SD rat is mated with SD male rats of 3 months old in a ratio of 2:1, a hydroxyurea distilled aqueous solution is administered from the third day of pregnancy to the 12th day of pregnancy to the rat, and the powder is dissolved in distilled water for administration to cause abortion; after abortion, from the next day of stopping of the vaginal bleeding of the rats, the rats are placed in a wooden barrel by batches at 14:00 every day, and the rats are forced to swim until the rats are fatigued by a wooden stick, and after swimming, the rats are removed; the rats are anesthetized under aseptic conditions, the endometrium is peeled off, an epithelial layer is reversed against a body wall, and the abdomen is closed, after 4 weeks, 3 rats are randomly taken and laparotomized, and the grafts are taken again for laparotomy to verify that the grafts are survived.The method establishes the SD rat model of EMs kidney deficiency and blood stasis syndrome, which makes the modeling of TCM syndrome experiment animals easy to control, and provides an accurate and effective way to identify syndrome characteristics.

Description

technical field [0001] The invention relates to the technical field of life sciences, in particular to a method for establishing a rat model of endometriosis with kidney deficiency and blood stasis syndrome. Background technique [0002] As an indispensable means for the development of modern life sciences, animal experiments have widely penetrated into various fields of basic medicine and clinical medicine of traditional Chinese medicine. The development of TCM animal models is based on the overall concept of TCM and the thinking of syndrome differentiation and treatment, using the theory of Zangxiang and etiology and pathogenesis to simulate some characteristics of human disease prototypes in animals. The replication of standardized TCM animal models is an important link in promoting the modernization of TCM and is of great significance to the development of TCM. [0003] However, the main problems at present are: it is difficult to control the experimental animal models ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00A61D7/00A61K31/17A61K31/567
Inventor 崔轶凡李培硕刘宏奇宗惠郝世凤曹娟
Owner SHANXI UNIV OF CHINESE MEDICINE
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