Preparation method of mesenchymal stem cell artificial pancreas islet

A technology of stem cells and pancreatic islets, applied in the field of biomedicine, can solve the problems of accelerating the vascularization of artificial pancreatic islets, the large number of pancreatic islets, and strong immune rejection.

Inactive Publication Date: 2019-06-07
山西宾大干细胞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention uses chitosan hydrogel compounded with islet-like cells induced by mesenchymal stem cells and vascular endothelial cells to prepare artificial islets, in order to solve the insulin resistance that occurs in the treatment of diabetes and the number of islets required for islet transplantation is relatively small. The problem of multiple and strong immune rejection, and the addition of vascular endothelial cells can accelerate the vascularization of artificial islets, make islet-like cells survive better, and help prolong the treatment time

Method used

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  • Preparation method of mesenchymal stem cell artificial pancreas islet

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Embodiment 1

[0120] A preparation method of mesenchymal stem cell artificial islets, comprising the following steps:

[0121] (1) Mesenchymal stem cells are cultured and induced to generate islet-like cells;

[0122] (2) Extraction and cultivation of vascular endothelial cells;

[0123] (3) Preparation of thermosensitive chitosan hydrogel;

[0124] (4) preparing composite hydrogel;

[0125] (5) After local transplantation of the composite hydrogel obtained in step (4), agglutination occurs rapidly in a body temperature environment of 37° C. to form artificial islets.

[0126] Further, the mesenchymal stem cells in step (1) are umbilical cord mesenchymal stem cells, which include:

[0127] (11) Extraction of umbilical cord mesenchymal stem cells

[0128] (111) Under aseptic conditions, take a healthy umbilical cord specimen of 10 cm and place it in 1% PBS buffer containing double antibodies;

[0129] (112) In the ultra-clean workbench, repeatedly rinse the umbilical cord specimen with PB...

Embodiment 2

[0167] A preparation method of mesenchymal stem cell artificial islets, comprising the following steps:

[0168] (1) Mesenchymal stem cells are cultured and induced to generate islet-like cells;

[0169] (2) Extraction and cultivation of vascular endothelial cells;

[0170] (3) Preparation of thermosensitive chitosan hydrogel;

[0171] (4) preparing composite hydrogel;

[0172] (5) After local transplantation of the composite hydrogel obtained in step (4), agglutination occurs rapidly in a body temperature environment of 37° C. to form artificial islets.

[0173] Further, the mesenchymal stem cells in step (1) are umbilical cord mesenchymal stem cells, which include:

[0174] (11) Extraction of umbilical cord mesenchymal stem cells

[0175] (111) Under aseptic conditions, take a healthy umbilical cord specimen of 20 cm and place it in 1% PBS buffer containing double antibodies;

[0176] (112) In the ultra-clean workbench, repeatedly rinse the umbilical cord specimen with ...

Embodiment 3

[0213] A preparation method of mesenchymal stem cell artificial islets, comprising the following steps:

[0214] (1) Mesenchymal stem cells are cultured and induced to generate islet-like cells;

[0215] (2) Extraction and cultivation of vascular endothelial cells;

[0216] (3) Preparation of thermosensitive chitosan hydrogel;

[0217] (4) preparing composite hydrogel;

[0218] (5) After local transplantation of the composite hydrogel obtained in step (4), agglutination occurs rapidly in a body temperature environment of 37° C. to form artificial islets.

[0219] Further, the mesenchymal stem cells in step (1) are umbilical cord mesenchymal stem cells, which include:

[0220] (11) Extraction of umbilical cord mesenchymal stem cells

[0221] (111) Under sterile conditions, take a healthy umbilical cord specimen of 15 cm and place it in 1% PBS buffer containing double antibodies;

[0222] (112) In the ultra-clean workbench, repeatedly rinse the umbilical cord specimen with ...

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Abstract

The invention relates to the field of biomedicine, in particular to a preparation method of a mesenchymal stem cell artificial pancreas islet. The preparation method of a mesenchymal stem cell artificial pancreas islet comprises the following steps of (1) culturing mesenchymal stem cells, and performing inducing to generate ancreas islet like cells; (2) performing extraction and culture of vascular endothelial cells; (3) preparing temperature-sensitive chitosan hydrogel; (4) preparing composite hydrogel; and (5) performing partial transplantation on the composite hydrogel obtained in the step(4), and rapidly performing agglutination under the 37 DEG C body temperature environment to form the artificial pancreas islet. According to the preparation method of the mesenchymal stem cell artificial pancreas islet disclosed by the invention, the problems of insulin resistance in the insulin treatment course, large number of pancreas islets required during pancreas islet transplantation and high immunological rejection reactions during pancreas islet transplantation are solved, and new donors are provided for pancreas islet transplantation for treating type I diabetes mellitus.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a preparation method of mesenchymal stem cell artificial islets. Background technique [0002] Diabetes mellitus (DM, Diabetes Mellitus) is a disorder of sugar, fat and protein metabolism caused by relative or absolute deficiency of insulin secretion or insulin receptor defect. Diabetes mellitus is an endocrine disease with serious complications, which greatly endangers human health. [0003] Type I diabetes is the absolute lack of insulin caused by the destruction of pancreatic beta cells. At present, there are two methods of insulin injection and islet transplantation for the treatment of type I diabetes, but both have limitations. Insulin injections are the most effective means of reducing the symptoms of diabetes. However, patients develop insulin resistance after injecting insulin for a period of time. The body will produce insulin antibodies, leading to a great loss of the bi...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/20A61L27/52C12N5/0775C12N5/071
Inventor 傅松涛郭璇
Owner 山西宾大干细胞生物科技有限公司
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