High-quality automatic roxburgh anoectochilus terminal bud breeding technology

Abstract

The invention provides a high-quality automatic roxburgh anoectochilus terminal bud breeding technology. A traditional roxburgh anoectochilus terminal bud breeding technical form is broken through, and protocorms subjected to chromosome doubling treatment are placed into a cluster culture medium and continue to be cultured to obtain clustered buds; the clustered buds are divided, transferred intoa rooting medium and cultured to obtain tissue culture seedlings with complete roots, stems and leaves; the tissue culture seedlings are screened to obtain polyploid plants; at least one robust targetpolyploid plant is selected from the polyploid plants; a plurality of target seedlings are cultured by the aid of the target polyploid plant; the target seedlings are bred and cultured in a multi-parameter variation manner by an intelligent breeding mechanism to obtain target roxburgh anoectochilus terminal bud seedlings; at least one strongly growing target roxburgh anoectochilus terminal bud seedling is selected, and parameters of the growth environment of the target roxburgh anoectochilus terminal bud seedling are recorded as standard growth environment parameters after a certain time. Therefore, the environment adapts to varieties, the optionally selected varieties are under optimal growth conditions, optimal growth quality is achieved, and breeding efficiency can be more effectivelyimproved.

Description

technical field [0001] The invention relates to the field of plant cultivation methods, in particular to a high-quality automatic cultivation process of Aromatica clematis. Background technique [0002] The breeding method of polyploid clematis is known, as Chinese invention patent CN201510749602.1 discloses a kind of breeding method of polyploid clematis kind, comprises the following steps: (a), the diploid clematis The seeds are cultivated to obtain sprouts; (b), the sprouts are soaked in a 5-15 μmol / L asulphin solution for 10-36 hours; (c), the soaked sprouts are continuously cultivated to obtain clustered sprouts; ( d), the clustered shoots are divided into rooting medium and then cultured to obtain tissue-cultured seedlings with complete roots, stems and leaves; (e), the tissue-cultured seedlings are screened to obtain polyploid plants. In step (a) and step (c), the cultivation is carried out using the first medium; the first medium is: MS medium containing BA 0.8-1.2m...

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Problems solved by technology

[0003] The steps of this invention are simple, but it is similar to other cultivation methods on the market, all are to fix the growth environment, and the species of clematis is selected, so that the species of clematis can adapt to the environment, because the environmental factors are many Diverse, more complex and variable than clematis varieties, and some environments may negatively affect clematis varieties, even if the most suitable variety is selected for the environment, it is actually not conducive to the clematis. Lotus grows, and if new clematis varieties are cultivated based on the environment, the cost will be greatly increased

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