Temperature control device and method used for nucleic acid reaction

Abstract

The invention discloses a temperature control device and method used for a nucleic acid reaction. The temperature control device comprises a reaction module, a temperature measuring module, a main control board, a control module, an electric heating module, a heat dissipation module and a display module. By using the device, the plurality of temperature sensors are used to monitor a temperature difference between a chip and a heating block in real time. The heating block in the device can be moved up and down and be rotated, and a distance between the heating block and the reaction module is increased and a variable speed fan and a cooling fin are used to realize cooling during a nucleic acid reaction process. The device and the method have the advantages of a fast heating speed, high precision, low cost and the like. Simultaneously, cooling can be controlled in real time, temperature overshoot is effectively prevented, and a rapid and uniform cooling process is realized. In addition,a plurality of reaction tanks included in the reaction module can simultaneously realize different nucleic acid reactions, and the reaction efficiency of the nucleic acid reactions is further increased.

Description

technical field [0001] The invention belongs to the field of biotechnology instruments, in particular to a temperature control device and method for nucleic acid reaction. Background technique [0002] Nucleic acid amplification technology currently includes conventional digital polymerase chain reaction technology, real-time fluorescent digital polymerase chain reaction technology and isothermal nucleic acid amplification technology, etc. Among them, digital polymerase chain reaction technology (Polymerase Chain Reaction, PCR for short) is a Molecular biology technology for amplifying specific DNA molecular fragments. It uses DNA molecules as templates, and uses a pair of artificially synthesized specific oligonucleotide primers to rapidly amplify specific DNA molecular fragments through the enzymatic reaction of DNA polymerase. biologically very important. The basic process of PCR reaction is divided into three steps. The first step is DNA denaturation (94°C), and the hy...

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Problems solved by technology

However, the metal coil used as a heater in this invention has high cost, is easily damaged, and is inconvenient to maintain. At the same time, the invention does not specify the specific heat dissipation method of the fan.

[0005] Aiming at the problems of temperature control accuracy and inaccurate control of heating and cooling speed in nucleic acid amplification technology, a temperature control device and method for nucleic acid reaction should be found, so that the device and method have fast and accurate heating speed, low cost, The advantages of low energy consumption and real-time control of cooling can effectively prevent temperature overshoot

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