Detection reagent kit optimized based on modified sphingomyelinase by HDL3 color comparison method and preparation and utilization method of detection reagent kit

A detection kit and sphingomyelinase technology, which can be used in biochemical equipment and methods, determination/inspection of microorganisms, resistance to vector-borne diseases, etc., can solve the problems of long time, high cost, and high technical requirements for operation

Active Publication Date: 2019-06-04
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the ultracentrifugation method uses the difference in specific gravity of lipoproteins to perform typing through centrifugation; its disadvantages are: high technical requirements for operation, high cost, and long time consumption
The disadvantage of the precipitation method is that the operation needs to be skilled, manual operation is required, and sometimes the pretreatment operation of the sample is required, which takes too long and is not universal.

Method used

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  • Detection reagent kit optimized based on modified sphingomyelinase by HDL3 color comparison method and preparation and utilization method of detection reagent kit
  • Detection reagent kit optimized based on modified sphingomyelinase by HDL3 color comparison method and preparation and utilization method of detection reagent kit
  • Detection reagent kit optimized based on modified sphingomyelinase by HDL3 color comparison method and preparation and utilization method of detection reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] A colorimetric detection kit for HDL3 based on modified sphingomyelinase optimization in this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0084] Reagent R1:

[0085]

[0086]

[0087] Reagent R2:

[0088]

[0089] In addition, HDL3 calibrator is also attached in the kit, and the components and corresponding contents included in the calibrator are as follows:

[0090]

[0091] Further, the Polyoxyethylene styreneated phenyl ether derivative is specifically EMULGEN B-66, a product of Kao Corporation.

[0092] Further, the Polyoxyethylene polycyclic phenyl ether is specifically NIKKOL BC-15, a product of Nikko Chemical Company.

Embodiment 2

[0094] A colorimetric detection kit for HDL3 based on modified sphingomyelinase optimization in this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0095] Reagent R1:

[0096]

[0097] Reagent R2:

[0098]

[0099]

[0100] In addition, HDL3 calibrator is also attached in the kit, and the components and corresponding contents included in the calibrator are as follows:

[0101]

[0102] Further, the Polyoxyethylene styreneated phenyl ether derivative is specifically NIKKOL BC-10, a product of Nikko Chemical Company.

[0103] Further, the Polyoxyethylene polycyclic phenyl ether is specifically NIKKOL BD-10, a product of Nikko Chemical Company.

Embodiment 3

[0105]A colorimetric detection kit for HDL3 based on modified sphingomyelinase optimization in this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0106] Reagent R1:

[0107]

[0108]

[0109] Reagent R2:

[0110]

[0111]

[0112] In addition, HDL3 calibrator is also attached in the kit, and the components and corresponding contents included in the calibrator are as follows:

[0113]

[0114] Further, the Polyoxyethylene styreneated phenyl ether derivative is specifically EMULGEN A-90, a product of Kao Corporation.

[0115] Further, the Polyoxyethylene polycyclic phenyl ether is specifically NIKKOL BO-15V, a product of Nikko Chemical Company.

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PUM

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Abstract

The invention provides a detection reagent kit optimized based on modified sphingomyelinase by an HDL3 color comparison method. The detection reagent kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises Tris-HCl, PEG6000, BSA, Polyoxyethylene styrenated phenyl ether derivative and the like, and the reagent R2 comprises Tris-HCl, PEG6000, mPEG-SMASE2, Polyoxyethylene polycyclic phenyl ether. The invention further provides a preparation and utilization method of the detection reagent kit optimized based on modified sphingomyelinase by the HDL3 color comparison method. The reagent kit can be used for analysis of a full-automatic biochemical analyser, is low in cost and high in automation, and can greatly save the detection time. Compared with like products, the reagent kit disclosed by the invention is higher in stability and sensitivity.

Description

technical field [0001] The invention relates to the field of biochemical determination and analysis, in particular to a modified sphingomyelinase-based optimized HDL3 colorimetric detection kit and a preparation method thereof. Background technique [0002] High-density lipoprotein 3 (High-density lipoprotein 3, HDL3) is one of the subcomponents of high-density lipoprotein (High-density lipoprotein, HDL). HDL is one of the main lipoproteins in plasma, with the highest density and the smallest particle diameter, and the main protein composition is apolipoprotein. HDL, phospholipids, apolipoproteins, cholesterol and a small amount of fatty acids form high-density lipoprotein cholesterol (High-density lipoprotein cholesterol, HDL-C), and the difference in the amount of bound cholesterol on HDL forms HDL1-C, HDL2-C and HDL3- The three subtypes of C correspond to the three lipoprotein subtypes of HDL1, HDL2 and HDL3, and there is a specific proportional relationship between them...

Claims

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Application Information

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IPC IPC(8): C12Q1/60C12Q1/44C12Q1/30C12Q1/28C12Q1/26
CPCY02A50/30
Inventor 符修乐芮双印吴瑶瑶陆大伟支新梅鲁萍高帆
Owner ANHUI DAQIAN BIO ENG LIMITED
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