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Screening method for lactic acid bacteria

A screening method, the technology of lactic acid bacteria, applied in the field of microbial screening, can solve the problems of cost waste, time-consuming, labor-intensive and labor-intensive determination of physical and chemical properties, so as to avoid the waste of manpower and materials, increase the accuracy of screening, and have strong pertinence Effect

Pending Publication Date: 2019-06-04
GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI +2
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Problems solved by technology

[0004] At present, many scientific researchers are engaged in the screening of lactic acid bacteria, but the current screening methods are mostly screening in large quantities first, and then measuring the physical and chemical properties. After the initial identification of lactic acid bacteria, 16s sequencing is carried out to determine the name of the bacteria, and then the antibacterial experiment is carried out. In this process, large-scale screening is already very labor-intensive, and the subsequent determination of physical and chemical properties is not only time-consuming and labor-intensive, but also a waste of cost

Method used

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  • Screening method for lactic acid bacteria
  • Screening method for lactic acid bacteria
  • Screening method for lactic acid bacteria

Examples

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Effect test

Embodiment 1

[0064] Vibrio harveyi (V.h) was used as the indicator bacteria to screen the bacterial strains with antibacterial effect, and it was poured on the MRS medium with bacteria and cultivated for 16h. The experimental results were as follows: figure 2 After a single colony is isolated, use a filter paper to verify the results as follows Figure 6 .

Embodiment 2

[0066] Use vibrio parahaemolyticus (Vibrioparahaemolyticus, V.p) as the indicator bacteria to screen the bacterial strains with antibacterial effect, pour it on the MRS medium with bacteria and cultivate it for 16h, the experimental results are as follows: image 3 After a single colony is isolated, use a filter paper to verify the results as follows Figure 7 .

Embodiment 3

[0068] Use Staphylococcus aureus (Staphylococcus aureus, S.a) as the indicator bacteria to screen the strains with antibacterial effect, pour it on the MRS medium with bacteria and cultivate it for 16h, the experimental results are as follows: Figure 4 After a single colony is isolated, use a filter paper to verify the results as follows Figure 8 .

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Abstract

The invention relates to the technical field of microbial screening, and in particular, relates to a screening method for lactic acid bacteria. The method includes the steps: grinding a sample firstly, and then carrying out enlarged cultivation; then growing on a culture medium by a tilt-pour process so as to separate single colonies of lactic acid bacteria; inoculating an MRS flat plate with indicator bacteria with single colonies by a bacteria dotting manner, and covering with MRS soft agar; selecting strains with a bacteriostatic effect, and carrying out purification and preservation of thesingle colonies; and finally, verifying. The strains separated and screened by the method not only can reduce the impact of other miscellaneous bacteria in early stage, but also can eliminate the non-bacteriostatic lactic acid bacteria, can reduce the workload, also can screen the required strains for inhibiting pathogenic bacteria more pertinently.

Description

technical field [0001] The invention belongs to the technical field of microbial screening, and in particular relates to a screening method for lactic acid bacteria. Background technique [0002] In recent years, with the continuous development of my country's economy, great changes have taken place in people's diet structure. Reasonable diet and balanced nutrition have become the main concerns of people. There are various varieties of aquatic animals, and they are a kind of food with very balanced nutrient content. They not only contain rich protein that is easy to absorb by the human body, but also have low fat content, and also have various vitamins and minerals needed by the human body, and are deeply loved by the majority of people. I love it, so my country's aquatic industry has developed very rapidly in recent years. In order to obtain high yields, people have gradually developed the aquaculture industry in the direction of high density and high intensification, but t...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 刘陈立刘明珠蒙海林何敬愉崔金明刘复荣杨金芳邓登
Owner GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI
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