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Preparation method for corn double haploid

A technology of double haploid and haploid immature embryos, applied in botanical equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problem of being easily affected by the environment and genotype, and not meeting the requirements of haploid breeding The need for large-scale application of technology, low frequency of natural doubling, etc., to achieve the effect of vigorous cell division, easy pollination operation, and large amount of loose powder

Active Publication Date: 2019-06-04
CHINA AGRI UNIV
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AI Technical Summary

Problems solved by technology

The frequency of natural doubling is often relatively low, and is easily affected by the environment and genotype, which is far from meeting the needs of large-scale application of haploid breeding technology

Method used

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  • Preparation method for corn double haploid
  • Preparation method for corn double haploid
  • Preparation method for corn double haploid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, a kind of preparation method of corn double haploid

[0051] 1. Obtaining maize haploid immature embryos

[0052] 1. Parent material and its planting

[0053] Maize parthenogenetic induction lines CAU5 and CHOI3 containing R1-nj markers were used as male parents, and the hybrids obtained by crossing B73 and Mo17, namely B73 / Mo17 (hereinafter referred to as "BM") were used as female parents.

[0054] The planting time and location of the parent materials are as follows: Hainan in the winter of 2014 and Beijing in the spring of 2015.

[0055] The planting method of the parent material is as follows: the parents are planted in a staggered period of about 1:6. In order to ensure that the parents meet at the flowering period and the pollination of the female parent is sufficient when the male parent is pollinated, the first female parent is matched with the third male parent.

[0056] 2. Pollination

[0057] The tassels of the female parent are removed befor...

Embodiment 2

[0109] Embodiment 2, the influence of seedling growth medium on the direct seedling growth of maize haploid immature embryos

[0110] 1. Obtaining maize haploid immature embryos

[0111] Same as Step 1 in Example 1, except that the parental materials were replaced with "the maize parthenogenetic inducible line CAU5 containing the R1-nj marker is used as the male parent, and the excellent maize hybrid Zhengdan 958 is used as the female parent".

[0112] 2. Identification and doubling of haploid immature embryos

[0113] Same as Step 2 in Example 1.

[0114] 3. After doubling, the haploid immature embryos directly grow into seedlings

[0115] The pseudo-haploid immature embryos selected in step 2 were respectively placed in D1-D6 medium for direct seedling cultivation, continuously cultivated for 9 days, and counted the number of leaves, roots, plant height and maximum height of the seedlings obtained in each medium respectively. long root length. The components and concentr...

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Abstract

The invention discloses a preparation method for corn double haploid. Based on a haploid young embryo identification technology, a haploid young embryo doubling technology and a young embryo direct seeding formation technology, a haploid tissue culture identification and doubling technology system can be established, the novel method for rapidly obtaining corn double haploid can be provided; and the method has characteristics of being rapid, efficient and easy in scale operation. Compared with haploid doubling conventional methods, time can be greatly shortened, the scale operation of tissue culture doubling can be more easily, and therefore, the method is suitable for demands of future haploid engineering seed breeding and has large application values in future haploid seed breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of corn double haploid (DH). Background technique [0002] Maize is a model crop that utilizes heterosis, and the most critical link is pure line selection. The breeding of conventional maize inbred lines requires multiple generations of self-selection, so it often takes several years to breed a variety; and theoretically, even if self-crossing n(n∝∞) generations, the genotype cannot 100% complete homozygosity is achieved. Using the parthenogenetic induction line to induce haploids and artificially double, only two generations can be used to obtain 100% homozygous inbred lines. Compared with conventional methods, it not only greatly shortens the breeding process, but also saves a lot of manpower and material resources. and financial resources. [0003] Common methods of doubling haploids include natural doubling and chemical doubling. The frequenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H1/02A01H1/04A01H4/00
Inventor 陈绍江陈琛钟裕陈宝建李金龙张俊稳祁晓龙
Owner CHINA AGRI UNIV
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