Cell extraction solution and application thereof

A technology for extracting liquid and cells, applied in the field of pharmacy, which can solve problems such as interference in doctor reading and diagnosis

Active Publication Date: 2019-05-24
孝感宏翔生物医械技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the TCT examination, doctors usually use a special cervical cell sampling brush to collect cells. During the collection process, the collected mucus and leucorrhea of...

Method used

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  • Cell extraction solution and application thereof
  • Cell extraction solution and application thereof
  • Cell extraction solution and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A cell extract, composed of the following mass fractions: glycerol 30%, potassium chloride 0.01%, potassium dihydrogen phosphate 0.02%, disodium hydrogen phosphate 0.15%, sodium chloride 0.8%, ethylenediaminetetraacetic acid di Sodium 0.6%, phenoxyethanol 0.1%, the remaining components are ultrapure water, and the specific gravity of the cell extract is 1.06.

Embodiment 2

[0049] A cell extract, composed of the following mass fractions: glycerol 35%, potassium chloride 0.01%, potassium dihydrogen phosphate 0.02%, disodium hydrogen phosphate 0.15%, sodium chloride 0.8%, ethylenediaminetetraacetic acid di Sodium 0.6%, phenoxyethanol 0.1%, the remaining components are ultrapure water, and the specific gravity of the cell extract is 1.07.

Embodiment 3

[0051] Sample experiment using the cell extract prepared in Example 1

[0052] 1. Preparation of relevant reagents:

[0053] 1.1 Buffer: prepare 10mM Tris buffer with pH 7.2.

[0054] 1.2 Rinsing solution: Mix absolute ethanol and isopropanol according to equal volumes.

[0055] 1.3 Papanicolaou staining solution: the Papanicolaou staining solution used in this example is the Papanicolaou staining solution produced by Xiaogan Hongxiang Biomedical Technology Co., Ltd.

[0056] 1.4 Liquid-based cell preservation solution sampling bottle: The liquid-based cell preservation solution used in this example is the liquid-based cell preservation solution produced by Xiaogan Hongxiang Biomedical Equipment Technology Co., Ltd.

[0057]2. Sample centrifugation and stratification: take an appropriate amount of centrifuge tubes and number them corresponding to the sample bottle of the liquid-based cell preservation solution, add 4 mL of the cell extract prepared in Example 1, and then tak...

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Abstract

The invention provides cell extraction solution and an application thereof, and belongs to the technical field of agents. The cell extraction solution comprises, by weight, 29-35 parts of glycerol, 0.005-0.015 part of potassium chloride, 0.01-0.03 part of monopotassium phosphate, 0.1-0.2 part of sodium hydrogen phosphate, 0.5-1 part of sodium chloride, 0.4-0.8 part of ethylene diamine tetraaceticacid, 0.05-0.15 part of phenoxyethanol and 63-69 parts of water. By the aid of the cell extraction solution, mucus and leucorrhea in a sample can be removed, squamous cells can be extracted, so that impurities and the squamous cells are separated, a doctor conveniently reads pieces and diagnoses, and the efficiency and the accuracy rate of a doctor in liquid-based cytological diagnosis.

Description

technical field [0001] The invention relates to the technical field of medicaments, in particular to a cell extract and its application. Background technique [0002] Cervical cancer, also known as cervical cancer, is a malignant tumor that occurs in the lining epithelium and glandular epithelium of the cervix. Cervical cancer is a common gynecological malignancy, and its incidence rate ranks second among female malignancies in recent years. [0003] At present, the commonly used cervical cancer screening is liquid-based cytology test (TCT test), which uses a liquid-based thin-layer cytology detection system to detect cervical cancer cells and perform cytological classification and diagnosis. In the TCT examination, doctors usually use a special cervical cell sampling brush to collect cells. During the collection process, the collected mucus and leucorrhea will affect the diagnosis doctor's interpretation of impurities due to the different lesions of individual patients. P...

Claims

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Application Information

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IPC IPC(8): C12N5/071G01N1/28G01N1/30G01N1/34G01N1/38G01N21/84
Inventor 赵彬沈红安
Owner 孝感宏翔生物医械技术有限公司
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