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Application of plant essential oil in inhibiting mycete in air

A plant essential oil, museum technology, applied in the fields of chemicals, applications, plant growth regulators for biological control, etc., can solve the problems of few preventive studies and no reports of fungal activity.

Active Publication Date: 2019-05-24
广西民族博物馆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, there are not many preventive studies on the use of plant essential oils to prevent and control biological diseases in museums, and there are no reports on the use of plant essential oils in museums to inhibit mold activity in cultural relics exhibition environments.

Method used

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  • Application of plant essential oil in inhibiting mycete in air
  • Application of plant essential oil in inhibiting mycete in air
  • Application of plant essential oil in inhibiting mycete in air

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] This embodiment is the application of the oregano essential oil in the described plant essential oils in inhibiting Penicillium oxalicum, Penicillium hockii and Aspergillus aculeatus for bacteriostasis, and the specific operation steps and control technical conditions are:

[0071] The plant essential oils described in this example include oregano essential oil, cinnamon essential oil and clove essential oil, all of which are commercially available. The plant essential oils are determined by gas chromatography-mass spectrometry, and each essential oil contains the following relative percentages of compounds: cinnamon Essential oil contains 70-85% cinnamaldehyde, 2-10% cinnamyl acetate, 2-7% coumarin, 1-5% benzaldehyde; oregano essential oil contains 50-60% carvacrol, 5-25% 5-methyl-2,4-diisopropylphenol, 1.5-10% thymol, 1-10% 3-methyl-4-isopropylphenol; clove essential oil contains 60-75% eugenol, 15-25 % caryophyllene, 1-5% humulene, 0.1-5% caryophyllene alcohol.

[0...

Embodiment 2

[0082] This embodiment is the application of cinnamon essential oil in the plant essential oils in inhibiting Penicillium oxalicum, Penicillium hockii and Aspergillus aculeatus, the specific operation steps and control technical conditions are: other conditions are the same as in Example 1 .

[0083] (1) Take a plate of freshly cultured Penicillium huckerii, gently wash the spores on the surface of the colony with sterile deionized water repeatedly, and place it in a 200r / min, 28°C constant temperature shaker for 30min to fully shake, and then use Hemocytometer for counting, and finally dilute the spore suspension to 10 5 ~10 6 CFU / mL, stored in a refrigerator at 4°C for no more than 7 days, for later use;

[0084] (2) Add 10 mL of spore suspension to 500 mL of the potato dextrose medium that has been cooled to 50° C., mix quickly, then pour into a sterile plate, and wait to stand still to solidify horizontally;

[0085] (3) Punch holes on the test plate with a sterilized h...

Embodiment 3

[0090] This embodiment is the application of clove essential oil in the described plant essential oils in inhibiting Penicillium oxalicum, Penicillium hockii and Aspergillus aculeatus, the specific operation steps and control technical conditions are: other conditions are the same as in Example 1 .

[0091] (1) Take a plate of freshly cultured Penicillium huckerii, gently wash the spores on the surface of the colony with sterile deionized water repeatedly, and place it in a 200r / min, 28°C constant temperature shaker for 30min to fully shake, and then use Hemocytometer for counting, and finally dilute the spore suspension to 10 5 ~10 6 CFU / mL, stored in a refrigerator at 4°C for no more than 7 days, for later use;

[0092] (2) Add 10 mL of spore suspension to 500 mL of the potato dextrose medium that has been cooled to 50° C., mix quickly, then pour into a sterile plate, and wait to stand still to solidify horizontally;

[0093] (3) Punch holes on the test plate with a steri...

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Abstract

The invention provides application of plant essential oil in inhibiting mycete in the air. The plant essential oil is characterized in that cinnamon essential oil is mainly prepared from cinnamyl aldehyde, cinnamyl acetate, coumarin and benzaldehyde; oregano essential oil is mainly prepared from carvacrol, 5-methyl-2,4-diisopropyl phenol, thymol and 3-methyl-4-isopropyl phenol; clove essential oilis mainly prepared from eugenol, caryophyllene, humulene and caryophyllenol. In order to fully investigate the effect of the plant essential oil on the inhibition of the mycete in the air in a museum, the three kinds of plant essential oil are used for performing inhibition tests on penicillium oxalicum, penicillium herquei and aspergillus aculeatus respectively, and the three kinds of plant essential oil are compounded to perform synergistic effect inhibition tests on the penicillium oxalicum, penicillium herquei and aspergillus aculeatus respectively. Tests show that the three kinds of plant essential oil have obvious antibacterial effects on the three kinds of mycete either alone or in combination and have good application potential as an antifungal agent in the air.

Description

technical field [0001] The invention relates to the technical field of mildew prevention and disinfection in museum exhibition halls, showcase microenvironments and warehouses, in particular to the application of plant essential oils in museums to inhibit mold in the air. Background technique [0002] The climate in the south is warm and humid, and the textile, paper, and wooden cultural relics collected by the museum are prone to mold, which pollutes the surface of the exhibited cultural relics and the glass of the showcase, seriously affecting the display of the exhibits and the safety of the cultural relics. The prevention and control of biological diseases of cultural relics can be achieved through temperature and humidity control, ventilation and clean environment to control the growth of biological diseases. Among the methods of preventing mildew of cultural relics in museums, fumigation is considered to be the most effective and thorough method. Common fumigation Agen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N35/02A01N37/02A01N43/16A01N35/04A01N31/08A01N31/04A01N31/16A01N27/00A01N31/06A01P1/00A01P3/00
Inventor 田双娥郑琳
Owner 广西民族博物馆
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