Preparation method of novel electrochemical biosensor for detecting monocyte chemoattractant protein-1

A chemoattractant protein and monocyte technology is applied in the field of preparation of a new electrochemical biosensor for detecting monocyte chemotactic protein-1, which can solve the problems of small current signal and insufficient sensitivity, and achieves improved sensitivity and detection range. , promote development, improve the effect of solid load

Active Publication Date: 2019-05-10
CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, electrochemical immunosensors still have disadvantages: the current signal is too small to meet the required sensitivity, and higher sensitivity allows us to obtain accurate results with less biological samples

Method used

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  • Preparation method of novel electrochemical biosensor for detecting monocyte chemoattractant protein-1
  • Preparation method of novel electrochemical biosensor for detecting monocyte chemoattractant protein-1
  • Preparation method of novel electrochemical biosensor for detecting monocyte chemoattractant protein-1

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Embodiment 1

[0036] Step 1. First, 3mL rGO-TEPA solution (1mg mL -1 ) sonicate for at least 30 minutes. Then, 3 mL Thi (0.5 mM) and 25 μL 1% HAuCl 4 The solution was added to the above rGO-TEPA solution and vigorously stirred at room temperature for 12 h. The resultant was then collected by centrifugation at 9000 rpm for 15 minutes and washed three times with ultrapure water. The product collected by centrifugation was dissolved in 1 mL of ultrapure water and stored in a 4°C refrigerator.

[0037] Step 2. Use 0.3 and 0.05 μm Al respectively 2 o 3 Polish the electrode into a mirror surface with powder, then ultrasonically ultrasonicate the electrode for 5 minutes each in the order of ultrapure water, absolute ethanol, and ultrapure water, and dry at room temperature for later use;

[0038] Step 3. Add 10 μL of electrode modification material reduced graphene oxide tetraethylenepentamine-thionine-gold nanoparticles (rGO-TEPA-Thi-Au) composite dropwise on the surface of the electrode, an...

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Abstract

The invention discloses a specific hypersensitive sandwich electrochemical immunosensor based on a novel reduced graphene oxide tetraethylenepentamine-thionine-gold nanoparticle (rGO-TEPA-Thi-Au) composite material and ruthenium-palladium-platinum trimetallic nanoparticles (RuPdPt TNPs). The specific hypersensitive sandwich electrochemical immunosensor is used for detecting a monocyte chemoattractant protein-1 (MCP-1) in human serum. Reduced graphene oxide tetraethylenepentamine (rGO-TEPA) contains a large amount of amino groups and significantly accelerates electron transfer. Thionine (Thi) molecules increase the adsorption capacity of negatively charged AuCl4<-> ions. Gold nanoparticles (AuNPs) in a nanocomposite can provide active sites for immobilization of biological materials. In addition, the RuPdPt TNPs have excellent catalytic properties for the reduction of H2O2, and Pt-NH2 can effectively capture antibodies. The specific hypersensitive sandwich electrochemical immunosensor has the advantages that the linear range is wide, sensitivity is high, specificity is high, detection is rapid, good reusability is achieved, and the specific hypersensitive sandwich electrochemical immunosensor can be used for measure the MCP-1 content in the human serum, and has great potential in clinical testing.

Description

Technical field: [0001] The invention relates to a preparation method and application of an electrochemical immunosensor for quantitative detection of monocyte chemoattractant protein-1 in clinical practice, especially based on the reduced graphene oxide tetraethylenepentamine-thionine-gold nanoparticle composite material and a three-metal ruthenium-palladium-platinum nanocomposite material as a biosensor prepared as a signal probe for detecting monocyte chemoattractant protein-1, belonging to the field of electrochemical detection. Background technique: [0002] Cardiovascular disease is the most common cause of death in developed countries, and atherosclerosis is the main cause of cardiovascular disease. Monocyte chemoattractant protein-1 (MCP-1), a member of the cysteine-cysteine ​​family, also known as cysteine-cysteine ​​chemokine ligand 2, is associated with cardiovascular The pathogenesis of disease is closely related. Elevated levels of MCP-1 in human serum can lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N33/531
Inventor 于超何俊琳毛巍然
Owner CHONGQING MEDICAL UNIVERSITY
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