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Magnetic bead suspension for nucleic acid purification and screening and application thereof

A suspension and magnetic bead technology, used in biochemical equipment and methods, microbial determination/inspection, DNA preparation, etc., can solve the problems of few magnetic beads, uneven purification recovery rate and quality, and achieve stable performance and cost. Low, high flexibility effects

Inactive Publication Date: 2019-05-10
BEIJING USCI MEDICAL LAB CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic companies have also emerged many imitators. At present, there are many kinds of magnetic bead brands, the actual purification recovery rate is uneven, and there are fewer magnetic beads with good fragment screening ability.

Method used

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  • Magnetic bead suspension for nucleic acid purification and screening and application thereof
  • Magnetic bead suspension for nucleic acid purification and screening and application thereof
  • Magnetic bead suspension for nucleic acid purification and screening and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 magnetic bead suspension (1)

[0040] This example provides a method for preparing a magnetic bead suspension. The specific formula is: carboxylated nano-magnetic beads with a particle diameter between 0.5-1.0 μm, and the final concentration in the magnetic bead suspension is 1.0 mg / mL, a final concentration of 15% (mass percentage, g / 100mL) polyethylene glycol PEG10000, a final concentration of 1mol / L sodium chloride, 5mmol / L ethylenediaminetetraacetic acid, 2mmol / L Tris-HCl , a mass volume ratio (g / 100mL) of 0.01% sodium N-lauroyl sarcosinate, a mass volume ratio of 0.5% Tween 20, and a solvent of water. After preparation, store at 2°C-8°C. In the preparation process of the magnetic bead suspension of the present invention, water, PEG, sodium chloride and other high-concentration or high-concentration components are added first, and other low-concentration components are subsequently added. There is no special requirement for the sequ...

Embodiment 2

[0041] The preparation of embodiment 2 magnetic bead suspension (2)

[0042] This embodiment provides a magnetic bead suspension, the specific formula is: the solvent is water, the carboxylated nano-magnetic beads with a particle diameter of 0.5 μm, the final concentration in the magnetic bead suspension is 5 mg / mL, and the final concentration 15% (mass percentage, g / 100mL) of polyethylene glycol PEG6000, a final concentration of 0.5mol / L of sodium chloride and a final concentration of 1mol / L of magnesium chloride, 1mmol / L of ethylenediaminetetraacetic acid, 6mmol / L of Tris-HCl, mass volume ratio of 0.05% (g / 100mL) sodium N-lauroyl sarcosinate, mass volume ratio of 0.5% 1% Tween 20. After preparation, store at 2°C-8°C.

Embodiment 3

[0043] The preparation of embodiment 3 magnetic bead suspension (3)

[0044] This embodiment provides a magnetic bead suspension, the specific formula is: the solvent is water, the carboxylated nano-magnetic beads with a particle diameter of 1 μm, the final concentration in the magnetic bead suspension is 1 mg / mL, and the final concentration is The polyethylene glycol PEG8000 of 20% (mass percentage), final concentration is the sodium chloride of 2.5mol / L and the magnesium chloride that final concentration is 1.0mol / L, the ethylenediaminetetraacetic acid of 5mmol / L, the Tris of 2mmol / L -HCl, sodium N-lauroyl sarcosinate with a mass volume ratio of 0.01%, and Tween 20 with a mass volume ratio (g / 100mL) of 0.5%. After preparation, store at 2°C-8°C.

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Abstract

The invention belongs to the technical field of magnetic bead application, and discloses a magnetic bead suspension for nucleic acid purification and screening and application thereof. The magnetic bead suspension comprises a carboxylated modified nano magnetic bead, PEG polyethylene glycol, sodium chloride and / or magnesium chloride, ethylenediaminetetraacetic acid, Tris-HCl, N-sodium lauroyl sarcosinate and Tween 20, a solvent is water, and the concentration of the nano magnetic bead is 0.1-5 mg / mL. The magnetic bead suspension can purify nucleic acid with a recovery rate of up to 96.42%, andhave a good DNA fragment screening function. The magnetic bead suspension has stable performance, is suitable for high-throughput sample operation, has low cost and high flexibility, and is suitablefor use in various workstations.

Description

technical field [0001] The invention belongs to the technical field of magnetic beads application, and in particular relates to a magnetic bead suspension used for nucleic acid purification and screening and its application. Background technique [0002] With the rapid development of genomics detection technology, especially the maturation of next-generation sequencing (NGS) technology, the need for high-throughput, rapid, high-sensitivity, and standardized experimental operations is becoming more and more urgent after it is applied to clinical diagnosis or medical research. The library construction step of NGS sequencing technology is a very time-consuming and labor-intensive task. The sample needs to be purified between the enzymatic reaction steps so that the next experiment can be carried out. [0003] In the needs of small-scale experiments or scientific research projects, nucleic acid purification can use silicon-based filter columns or filter membranes to filter the s...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12Q1/6806
Inventor 孙广欣王冬王建伟伍启熹刘倩刘珂弟唐宇
Owner BEIJING USCI MEDICAL LAB CO LTD
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