Method for producing rabies vaccine by CEF cells suitable for sheet carrier bags

Abstract

The invention discloses a method for producing rabies vaccine by CEF cells suitable for sheet carrier bags, which comprises the following steps of: (1) preparing CEF cells; (2) primary culturing CEF cells in sheet carrier bags; (3) accessing rabies vaccine virus; (4) harvesting rabies vaccine virus solution to obtain rabies vaccine stock solution. According to the method, primary CEF cells cultureis directly carried out by adopting sheet carrier bags, and then rabies virus is inoculated, so that primary CEF cells required by large-scale culture can be cultured, and at the beginning of the production of rabies vaccine virus, namely SPF chicken embryos can be purchased directly and processed for current use at the beginning of cell culture without creating a database, the operation is convenient, time and labor are saved; and the problem of DNA residue of host cells is solved by adopting primary CEF cells to produce rabies virus vaccine. In addition, sheet carrier bags are used for carrying out mass transfer and oxygen transfer by swinging back and forth or left and right, and the static and dynamic combination of the swinging bed in the culture process is combined, so that the masstransfer and oxygen transfer efficiency is high.

Description

technical field [0001] The invention relates to the field of life sciences, especially cell culture and virus propagation. Specifically, it relates to a method for producing rabies vaccine from CEF cells suitable for sheet carrier bags. Background technique [0002] At present, most of the equipment used in the production of rabies vaccines and other products by CEF cells in the market is produced by glass or stainless steel bioreactors. However, when using glass and stainless steel bioreactors for production, a large number of spinner bottle passaging and digestion work and a large space are required in the early stage of cell proliferation and seed cell preparation. The production of the sheet carrier bag is to use a control system to control a swing bed reactor, place the sheet carrier bag on the swing bed, connect the gas control pipeline, and connect the cells with a square bottle, so that the cells can be placed in the swing bed. High-density proliferation and inocul...

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Problems solved by technology

[0003] In addition, when the existing microcarriers are used for cell culture, tidal movement is often used for mass transfer and oxygen transfer. It is difficult to add pancreatin to internal digestion; the tidal reactor of Yisco company transfers mass and oxygen through ebb and flow. It pumps the liquid out through a peristaltic pump, and then another peristaltic pump pumps the liquid in. in; it is a static exchange, less efficient than dynamic

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