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Laser microporous acellular dermal matrix and preparation method thereof

A technology of decellularized dermis and dermal matrix, which is applied in the field of preparation of new laser microporous acellular dermal matrix, can solve problems such as unsatisfactory repair effect, uneven distribution, and shrinkage of wound surface, and achieve good bio-histocompatibility, Micropore distribution is even and fine, and the effect of reducing the formation of scars

Inactive Publication Date: 2019-05-10
罗旭
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the mesh of the dermal matrix is ​​too large, and the distribution is not uniform enough, resulting in shrinkage of the wound surface and unsatisfactory repair effect

Method used

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  • Laser microporous acellular dermal matrix and preparation method thereof
  • Laser microporous acellular dermal matrix and preparation method thereof
  • Laser microporous acellular dermal matrix and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: picosecond laser processing

[0036] A preparation method for microporous allogeneic / heterogeneous acellular dermal matrix processed by a picosecond laser, comprising the following steps:

[0037] A. Set the parameters of the picosecond laser: the energy of each pulse is 150 microjoules, the pulse width is 8 picoseconds, the processing speed is 2mm / s, the attenuation range is 20%, the processing method is the dot method, and the spacing is 1 mm, dot twice at the same place continuously, the beam spot diameter of the laser is about 30 microns. Adjust the height of the light exit hole of the laser so that the focal plane falls on the dermal matrix to be processed;

[0038] B. Take out a sample of allogeneic / heterogeneous acellular dermal matrix from sterile water for injection, which is a 5 cm * 5 cm square, and quickly fix it on the processing platform of the laser processing equipment by four-point suspension fixation method; four points The suspension f...

Embodiment 2

[0045] Embodiment 2: nanosecond laser processing

[0046] The preparation method of microporous allogeneic / heterogeneous acellular dermal matrix processed by nanosecond laser comprises the following steps:

[0047] A. Set the parameters of the nanosecond laser processing equipment: the energy of each pulse is 2 millijoules, the pulse width is 2 nanoseconds, the processing speed is 5mm / s, the processing method is the dot method, and the spacing is 2mm. One place is marked once, and the beam spot diameter of the laser is about 50 microns. Adjust the height of the light exit hole of the laser so that the focal plane falls on the dermal matrix to be processed;

[0048] B. Take out a sample of allogeneic / heterogeneic acellular dermal matrix from sterile water for injection, which is a 5 cm * 5 cm square, quickly spread the sample on the sapphire substrate, and fix the edge on the substrate with plastic nails , the surface of the sapphire substrate close to the sample is a rough s...

Embodiment 3

[0059] A laser microporous allogeneic / heterogeneous acellular dermal matrix is ​​used for animal experiments of skin transplantation, the experimental animals are SPF grade SD rats, comprising the following steps:

[0060] A. Take as figure 1 Dorsal dermal tissues of decellularized small allogeneic / xenogeneic animals are shown:

[0061] B, according to the method of embodiment 1 or embodiment 2, prepare laser microporous allogeneic / heterogeneous dorsal dermal matrix, such as figure 2 As shown, the laser microporous allogeneic / heterogeneous dorsal dermal matrix is ​​soaked in a container filled with sterile water for injection;

[0062] C. Observed under a light microscope figure 2 Laser microporous allogeneic / xenogeneic acellular dermal matrix in such as image 3 shown;

[0063] D. Take a piece such as figure 2 The indicated laser microporous allograft / xenogeneic dorsal dermal matrix for subcutaneous burial experiments, Figure 4 It is shown that it exhibits good bioc...

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Abstract

The invention discloses a laser microporous acellular dermal matrix and a preparation method thereof. The preparation method uses a picosecond laser for puncturing an acellular dermal matrix to prepare a microporous acellular dermal matrix. The preparation method includes the following steps that the parameters of the picosecond laser are set, wherein each pulse energy is 150 microfocals, and thepulse width is 8 picoseconds; a sample is fixed on a processing platform of a pulse laser, and the upper and lower surfaces of the sample are kept wet; the picosecond laser is used for puncturing thesample; an ultrasonic machine is used for ultrasound treatment of the sample for 1-5 seconds; the sample is soaked in sterile water for injection. The preparation method makes the pore size of the laser-processed dermal matrix controllable, the edge is smooth, the micropore distribution of the prepared microporous acellular dermal matrix is uniform and fine, it is ensured that basal exudate of a wound can better nourish the skin through uniform meshes at the time of transplanting, the regeneration of blood vessels is promoted, the survival rate of skin graft is improved, and the formation of scars is reduced.

Description

[0001] This application is a divisional application of a patent application with an application date of August 8, 2014, an application number of 201410386574.7, and an invention title of "Laser Microporous Acellular Dermal Matrix and Its Preparation Method". technical field [0002] The invention relates to medical skin tissue engineering technology, in particular to a preparation method of a novel laser microporous decellularized dermal matrix. Background technique [0003] With the improvement of people's living standards in our country, the requirements for the treatment of acute and chronic skin wounds are also getting higher and higher. Ulcers caused by bedsores, varicose veins, vasculitis, diabetes, etc., and skin defects caused by acute trauma such as burns often make it difficult to repair wounds due to the lack of transplantable autologous skin grafts. Patients also face metabolic disorders caused by skin defects, Threats of pain, infection, sepsis and even death. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/56A61L27/60
Inventor 罗旭许金通
Owner 罗旭
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