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Application of peptide SLE2018-V001 in the diagnosis of systemic lupus erythematosus kit

A technology of SLE2018-V001 and diagnostic kit, which is applied in the field of biomedicine to achieve high sensitivity, assist early diagnosis of systemic lupus erythematosus, and high specificity and sensitivity

Active Publication Date: 2019-05-07
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many similar works, but the screening of biomarkers of systemic lupus erythematosus through random peptide library has not been reported. Therefore, we use high-throughput phage display library technology to screen peptides with high discrimination between patients and normal people , in order to obtain more effective early diagnostic markers for systemic lupus erythematosus

Method used

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  • Application of peptide SLE2018-V001 in the diagnosis of systemic lupus erythematosus kit
  • Application of peptide SLE2018-V001 in the diagnosis of systemic lupus erythematosus kit
  • Application of peptide SLE2018-V001 in the diagnosis of systemic lupus erythematosus kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Prepare the peptide and couple the peptide to BSA

[0047] 1) The polypeptide SLE2018-V001 (amino acid sequence: YEHAMYRSAVLL) is a polypeptide with a cysteine ​​linked to its C-terminus synthesized by Jill Biochemical (Shanghai) Co., Ltd. by HPLC. SMCC (succinimidyl cyclohexane-1-carboxylate) was purchased from Thermo (Cat. No. 22360). Dissolve in DMSO (dimethyl sulfoxide) to a final concentration of 100 mg / mL, and store at -20°C. The shelf life is one month.

[0048] 2) Weigh 10 mg of BSA and add 1 mL of coupling buffer PBS to prepare a 10 mg / mL BSA solution.

[0049] 3) Take 2mg of SMCC (the relationship with BSA is 1:5), add it to the above carrier protein solution, mix well, and react at 25°C for 1h.

[0050] 4) In a 10KD dialysis bag, dialyze overnight.

[0051] 5) Place the dialyzed carrier protein solution in a new centrifuge tube, add the original coupling buffer, and dilute the activated BSA-SMCC concentration to 4 mg / mL.

[0052] 6) Take 1 mg of the p...

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Abstract

The invention discloses a polypeptide SLE2018-V001 for use in diagnosing a systemic systemic lupus erythematosus kit. The amino acid sequence of the polypeptide SLE2018-V001 is: YEHAMYRSAVLL. The invention adopts the enzyme linked immunosorbent assay (ELISA) technology widely used in clinical practice, and uses an indirect method to qualitatively detect the level of IgG antibody against anti-YEHAMYRSAVLL polypeptide in human serum. The kit of the present invention can greatly improve the specificity and sensitivity of the early diagnosis of the systemic lupus erythematosus as an aid in the early diagnosis of the systemic lupus erythematosus, with a specificity of 74% and a sensitivity of 88%.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the application of a polypeptide SLE2018-V001 in a kit for diagnosing systemic lupus erythematosus. Background technique [0002] Systemic lupus erythematosus (SLE) is an autoimmune-mediated autoimmune disease (autoimmune diseases, ADs) clinically manifested as multi-system damage, often causing irreversible damage to multiple organ systems, seriously affecting the patient's longevity and quality of life. The incidence of systemic lupus erythematosus disease ranges from 0.02% to 0.07%, and the incidence rate of urban population is higher. The age of patients is mainly distributed between 15 and 45 years old, and most of them are female patients. The incidence ratio of male to female is about 1:9 (Lupus 2006.15(5):308–318.). The prevalence, pathological mechanism and biomarkers of systemic lupus erythematosus are three important aspects of systemic lupus erythematosus resea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/535G01N33/564
Inventor 陶生策吴凡林祁环李华赖丹昀胡传圣赵小东沈南唐元家郭强丁慧华
Owner SHANGHAI JIAO TONG UNIV
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