Cysteamine modified gold nanorod method and substrate and method for detecting acephate

Abstract

The invention especially relates to a cysteamine modified gold nanorod method. The method comprises the following steps of (A) taking a gold nanorod solution and washing, adding ultrapure water afterwashing and mixing, wherein the length of gold nanorods in the gold nanorod solution is 60-70 nm and a width is 20-23 nm; (B) mixing the washed gold nanorod solution and a cysteamine solution according to the volume ratio of 9:1 and then putting into a volumetric flask containing a magneton, wherein the concentration of the cysteamine solution is 10-6mol/L; (C) putting the volumetric flask on a blender and stirring for 60 to 90 minutes; and (D) after stirring, absorbing a cysteamine-modified gold nanorod solution and putting into a centrifugal machine for centrifugation, and then extracting asupernatant liquid to obtain cysteamine-modified gold nanorods. The invention also discloses a substrate and a method for detecting acephate. The cysteamine-modified gold nanorods are stable and uniform in form, and an enhancement effect is better. Simultaneously, an absorption effect exists between amine ions on gold nanorods and acephate molecules so as to realize the high sensitivity detectionof acephate residues.

Description

technical field [0001] The invention relates to the technical field of SERS detection, in particular to a method for modifying gold nanorods with cysteamine and a substrate and method for detecting acephate. Background technique [0002] Acephate is a slow-acting insecticide with stomach poisoning and contact killing effects, suitable for various chewing and sucking oral pesticides in vegetables, tea trees, tobacco, fruit trees, cotton, rice, wheat, rapeseed and other crops. Control of organ pests, mites and sanitation pests. Excessive, prolonged and unreasonable application methods can easily cause acephate residues in crops to exceed the standard. Traditional detection methods such as gas chromatography-mass spectrometry and high-performance liquid chromatography have high precision, but the pretreatment is cumbersome, time-consuming and expensive, and is not suitable for solving widespread residue problems. Surface-enhanced Raman spectroscopy (SERS for short) has great ...

AI Technical Summary

Problems solved by technology

As a molecule with a small Raman scattering cross section, acephate has a weak Raman response, and conventional substrates such as gold nanoparticles, gold nanorods, and silver nanoparticles have no obvious enhancement effect on it.

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