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Method for cultivating sargassum thunbergii for adsorbing metal ions in waste water

A technology of metal ions and salvia, which is applied in the field of marine organisms to achieve the effects of promoting expression, reducing work costs and increasing expression

Active Publication Date: 2019-05-03
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Now, it is difficult to find large areas of wild sargassum in the intertidal zone

Method used

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  • Method for cultivating sargassum thunbergii for adsorbing metal ions in waste water
  • Method for cultivating sargassum thunbergii for adsorbing metal ions in waste water
  • Method for cultivating sargassum thunbergii for adsorbing metal ions in waste water

Examples

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Effect test

Embodiment 1

[0032] The method for cultivating sargassum for absorbing metal ions in wastewater, including providing explants, then cultivating adventitious buds from the explants under irradiation conditions, and then multiplying the adventitious buds; the above irradiation conditions including electromagnetic waves. This method can complete the large-scale cultivation of Sargassum seedlings through tissue culture, and due to the increase in the expression of cyclin-related genes during the cultivation process, sprouts with more side branches are formed, and the attachment of seedlings to the attachment base The capacity and tolerance to heavy metal ions have been strengthened to obtain Sargassum seedlings and plants with stronger adsorption and removal capabilities.

[0033] Implants were prepared from sargassum fixers. Sargassum is a perennial large seaweed. The fixator attached to the reef can continuously germinate new algae, and clusters of young shoots grow on the fixer, so the fix...

Embodiment 2

[0043] The method for cultivating sargassum for adsorbing metal ions in waste water, the specific steps are as follows:

[0044] 1) After cleaning the sargassum fixer, disinfect it with alcohol with a volume concentration of 80% for 15 seconds, then disinfect it with a povidone-iodine solution with a volume concentration of 2% for 15 seconds, and then disinfect it with a sodium hypochlorite solution with a volume concentration of 2% for 15 minutes , washed with sterilized seawater, then placed in the antibiotic culture solution comprising 0.2g streptomycin, 20mg neomycin and 1.5mg nystatin, at 22°C of temperature, 1500lx of light intensity, light cycle light:dark=14: Under the condition of 10h, raise temporarily for 2 days;

[0045] 2) In the ultra-clean workbench, take the fixer out of the antibiotic culture solution, rinse it with sterilized seawater, and then transfer it to a sterilized surface dish, cut off the bleached epidermis around the fixer with a knife, and place th...

Embodiment 3

[0049] The method for cultivating sargassum for adsorbing metal ions in waste water, the specific steps are as follows:

[0050] 1) After cleaning the sargassum fixer, disinfect it with alcohol with a volume concentration of 80% for 15 seconds, then disinfect it with a povidone-iodine solution with a volume concentration of 2% for 15 seconds, and then disinfect it with a sodium hypochlorite solution with a volume concentration of 2% for 15 minutes , washed with sterilized seawater, then placed in the antibiotic culture solution comprising 0.15g streptomycin, 15mg neomycin and 0.5mg nystatin, at a temperature of 20°C, light intensity 1500lx, light cycle light: dark=14: Under the condition of 10h, raise temporarily for 3 days;

[0051] 2) In the ultra-clean workbench, take the fixer out of the antibiotic culture solution, rinse it with sterilized seawater, and then transfer it to a sterilized surface dish, cut off the bleached epidermis around the fixer with a knife, and place t...

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Abstract

The invention provides a method for cultivating sargassum thunbergii for adsorbing metal ions in waste water, and belongs to the technical field of marine organisms; the method comprises the followingsteps: providing an explant formed by a sterilized and temporarily cultured fixator, culturing adventitious buds of the explant under an intermittent irradiation condition, then carrying out propagation and cultivation on the adventitious buds under the condition of combination of flowing cultivation and domestication culture, wherein the irradiation condition includes electromagnetic waves. Themethod for cultivating the sargassum thunbergii for adsorbing the metal ions in the waste water can obtain the sargassum thunbergii seedlings which have increased soluble sugar content in cells, improved stress resistance and transplanting survival rate, enhanced adhesion capacity and metal ion tolerance and can grow air bags and reproductive supports in advance; during cultivation, the explants can form filamentous bodies and cell clusters and then develop into buds with more lateral branches, the adventitious bud generation rate is increased, the seedling yield is large, germplasm preservation and cyclic culture can be carried out for a long time, wild algae plants do not need to be collected, and the working cost is reduced, thereby achieving the purpose of artificial mass propagation and cultivation.

Description

technical field [0001] The invention belongs to the technical field of marine biology, and in particular relates to a method for cultivating sargassum for absorbing metal ions in waste water. Background technique [0002] In our country, the discharge of waste water is increasing day by day with the rapid improvement of industrialization level, resulting in serious environmental pollution problems. Heavy metals are one of the important causes of environmental pollution. When heavy metals enter the environment, they cannot be biodegraded into harmless substances. At the same time, suspended particles in the water absorb and precipitate heavy metal ions, making the water polluted by heavy metals for a long time. And so it came about. What's more, the heavy metals that exist in living organisms are accumulated and enriched, and they also participate in the cycle of the biosphere, endangering human health, and are a type of pollutant that is extremely harmful to the ecological ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G33/00
CPCY02A40/80
Inventor 景斐张建设丁月晗
Owner ZHEJIANG OCEAN UNIV
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