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Method for detecting dormancy degree of seed of phellodendron amurense Rupr. at constant temperature and application thereof

A seed and degree technology, applied in the field of constant temperature detection of the dormancy degree of Phellodendron chinensis seeds, can solve the problems that little attention is paid to the degree of seed dormancy, differences, etc., and achieve the effect of avoiding the increase of experimental cost, simple operation and ensuring accuracy.

Inactive Publication Date: 2019-04-30
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the research on seed dormancy is focused on whether the seeds have dormancy phenomenon and the release of dormancy, and little attention has been paid to the difference in the degree of seed dormancy

Method used

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  • Method for detecting dormancy degree of seed of phellodendron amurense Rupr. at constant temperature and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The present embodiment provides a kind of method that constant temperature detects the dormancy degree of Phellodendron amurense seed, specifically as follows:

[0041] 1. Take the newly harvested Phellodendron amurense fruit, peel off the Phellodendron amurense seeds from the fruit, wash, dry, and store at room temperature for more than 30 days;

[0042]2. Soak the Phellodendron amurense seeds in water for 24 hours, put them into low temperature treatment at 4°C and set up 5 repeated groups, each with 100 seeds;

[0043] 3. With the seed coat crack, the white radicle is visible as the seed germination sign. From the 45th day, observe and record the number of germinated seeds every 15 days, and take out the germinated seeds until more than 90% of the seeds germinate.

[0044] The detection result of present embodiment gained is shown in Table 1:

[0045] Table 1

[0046]

45 days

60 days

75 days

90 days

repeat group 1

30 capsules

...

Embodiment 2

[0051] The present embodiment provides a kind of method that constant temperature detects the dormancy degree of Phellodendron amurense seed, specifically as follows:

[0052] 1. Take the newly harvested Phellodendron amurense fruit, peel off the Phellodendron amurense seeds from the fruit, wash, dry, and store at room temperature for more than 30 days;

[0053] 2. Soak the Phellodendron amurense seeds in water for 24 hours, place them in a low-temperature treatment at 6°C and set up 5 repetition groups, with 100 seeds in each group;

[0054] 3. With the seed coat crack, the white radicle is visible as the seed germination sign. From the 45th day, observe and record the number of germinated seeds every 15 days, and take out the germinated seeds until more than 90% of the seeds germinate.

[0055] The detection result of present embodiment gained is shown in Table 2:

[0056] Table 2

[0057]

45 days

60 days

75 days

90 days

repeat group 1

2...

Embodiment 3

[0061] The present embodiment provides a kind of method that constant temperature detects the dormancy degree of Phellodendron amurense seed, specifically as follows:

[0062] 1. Take the newly harvested Phellodendron amurense fruit, peel off the Phellodendron amurense seeds from the fruit, wash, dry, and store at room temperature for more than 30 days;

[0063] 2. Soak the Phellodendron amurense seeds in water for 32 hours, place them in a low-temperature treatment at 4°C and set up 5 repetition groups, with 100 seeds in each group;

[0064] 3. With the seed coat crack, the white radicle is visible as the seed germination sign. From the 45th day, observe and record the number of germinated seeds every 15 days, and take out the germinated seeds until more than 90% of the seeds germinate.

[0065] The detection result of present embodiment gained is shown in Table 3:

[0066] table 3

[0067]

45 days

60 days

75 days

90 days

repeat group 1

2...

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Abstract

The invention belongs to the field of plant plantation, and specifically relates to a method for detecting a dormancy degree of a seed of phellodendron amurense Rupr. at constant temperature and an application thereof. The method comprises the following steps: soaking seeds of the phellodendron amurense Rupr. in water for 18-32 hours, then placing the seeds at a temperature of 0-6 DEG C for performing low temperature treatment, and arranging repeating groups of not less than 50 seeds per group; and splitting the seeds, regarding visible white radicle as marks of seed germination, starting fromthe 45th day, observing and recording the number of germinated seeds every 12-16 days, and taking out the germinated seeds until more than 90% of the seeds are germinated or the newly added germination rate is less than 1% for 30 consecutive days. The invention provides an efficient and accurate method for detecting the dormancy degree of the seed of the phellodendron amurense Rupr. at the constant temperature, and the method plays a practical and effective guiding role for a dormancy mechanism of the seeds of the phellodendron amurense Rupr., a seed dormancy release method, and optimizationof a cultivation strategy, and the like, thereby further promoting technology popularization and survival rate guarantee for artificially planting the phellodendron amurense Rupr..

Description

technical field [0001] The invention belongs to the field of plant planting, and in particular relates to a method for constant temperature detection of the dormancy degree of amurberry seeds and an application thereof. Background technique [0002] Phellodendron amurense is the main associated tree species of broad-leaved Korean pine forest in Northeast my country, and it is a national second-class protected plant. It is one of the three famous hardwoods in Northeast my country and one of the three medicinal woody plants in China. It integrates medicinal, material and ornamental value, and has a high prospect of protection, development and utilization. Due to a large number of felling and destruction of the ecological environment, the resource damage is very serious and is in a state of exhaustion. The restoration of wild resources of Phellodendron amurense is very difficult due to the barriers to self-renewal of the population and the physiological post-ripening character...

Claims

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Application Information

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IPC IPC(8): A01C1/02A01G17/00
CPCA01C1/025A01G17/005
Inventor 张昭段学伟张本刚齐耀东索凤梅徐硕范永芳
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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