Preservation solution and preparation process and application method thereof

Abstract

The invention discloses a preservation solution and a preparation process and application method thereof. The preservation solution is prepared from, by weight, 1.45-1.6 parts of sodium dihydrogen phosphate dihydrate, 2.58-2.7 parts of disodium hydrogen phosphate heptahydrate, 2.36-2.5 parts of EDTA disodium, 8.1-9 parts of sodium chloride, 3-4 parts of dithiothreotol, 8.1-9 parts of NH4Cl, 1,000mL of deionized water and a pH regulator, and the pH value of the preservation solution is 6.8-7.2. According to the preservation solution, cell mucus and fresh blood carried in cell samples in the sampling process can be effectively moved, and then effective cells are fully released; in the preservation process, the cell mucus and fresh blood are not likely to affect the effective cells, and thefinally obtained detection results can achieve the accurate effect.

Description

technical field [0001] The invention relates to the technical field of cell preservation solution, more specifically, it relates to a preservation solution and its preparation process and application method. Background technique [0002] Prevention of cervical cancer has become a global public issue. When detecting cervical cancer, the cells must be sampled first. At this time, some fresh blood and cell mucus will inevitably be brought out of the taken out cell samples, or some fresh blood cells may be mixed in the taken out cell samples. During the preservation process, although the traditional preservation solution can maintain a relatively stable pH value, the cell mucus, fresh blood, etc. mixed with the sample cells contain a relatively high content of protein that is difficult to remove, and even effectively removes some of the sample cells. cells encased inside. During the preservation process, the traditional preservation solution is difficult to remove the cell muc...

AI Technical Summary

Problems solved by technology

During the preservation process, although the traditional preservation solution can maintain a relatively stable pH value, the cell mucus, fresh blood, etc. mixed with the sample cells contain a relatively high content of protein that is difficult to remove, and even effectively removes some of the sample cells. cells encased in

During the preservation process, the traditional preservation solution is difficult to remove the cell mucus and fresh blood only through the stable pH value, so that it is difficult to fully release the effective cells covered by the cell mucus and fresh blood. In the follow-up detection, the number of effective cells is less than the actual number of effective cells, which eventually leads to inaccurate detection results

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