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Synthesis and application of reconfigurable molecular logic gate based on magnetic nanospheres

A magnetic nanosphere and magnetic nanoparticle technology, applied in the field of optical molecular logic gates of analytical chemistry, can solve the problem of lack of reset function of molecular logic sensing system and so on

Pending Publication Date: 2019-04-26
TAIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only a few molecular logic sensing systems can only complete the detection of no more than 2 targets, and the molecular logic sensing system lacks a reset function, and cannot perform the next round of analysis after the detection is completed.

Method used

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  • Synthesis and application of reconfigurable molecular logic gate based on magnetic nanospheres
  • Synthesis and application of reconfigurable molecular logic gate based on magnetic nanospheres
  • Synthesis and application of reconfigurable molecular logic gate based on magnetic nanospheres

Examples

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Effect test

Embodiment 1

[0026] Construction of an OR logic gate based on magnetic nanospheres and fluorescently labeled DNA: Magnetic nanospheres (MB1, MB2 and MB3) modified with molecular beacons and hairpin DNA (H 1 , H 2 and H 3 ) as a molecular logic sensing platform, adding different combinations of miR-21, miR-155 and miR Let-7a to construct OR logic gates. When the nucleic acid sequence (miR-21, miR-155 and miR Let-7a) was added, the input was recorded as "1", otherwise it was "0". Based on whether the relative fluorescence intensity value is greater than 0.3 or not, when the relative fluorescence intensity is greater than 0.3, the output is "1", and when the relative fluorescence intensity is less than 0.3, the output is "0". When there is no input, the molecular beacon is in a closed state, and the fluorescence of the entire computing system is also at a low value. The relative fluorescence intensity is less than 0.3, and the output is recorded as "0". The three miRNAs miR-21, miR-155 and...

Embodiment 2

[0028] Construction of INHIBIT logic gates based on miR-21 and P1: magnetic nanospheres (MB1, MB2 and MB3) modified with molecular beacons and hairpin DNA (H 1 , H 2 and H 3 ) as a molecular logic sensing platform, adding different combinations of miR-21 and P1 to construct INHIBIT logic gates. When nucleic acid sequences (miR-21 and P1) were added, the input was recorded as "1", otherwise "0". Based on whether the relative fluorescence intensity value is greater than 0.3 or not, when the relative fluorescence intensity is greater than 0.3, the output is "1", and when the relative fluorescence intensity is less than 0.3, the output is "0". When there is no input, the molecular beacon is in a closed state, and the fluorescence of the entire computing system is also at a low value. The relative fluorescence intensity is less than 0.3, and the output is recorded as "0". Since miR-21 can trigger molecular beacon (MB1) and hairpin probe (H 1 ), the molecular beacon is turned on...

Embodiment 3

[0030] Construction of INHIBIT logic gates based on miR-155 and P2: magnetic nanospheres (MB1, MB2 and MB3) modified with molecular beacons and hairpin DNA (H 1 , H 2 and H 3 ) as a molecular logic sensing platform, adding different combinations of miR-155 and P2 to construct INHIBIT logic gates. When nucleic acid sequences (miR-155 and P2) were added, the input was recorded as "1", otherwise "0". Based on whether the relative fluorescence intensity value is greater than 0.3 or not, when the relative fluorescence intensity is greater than 0.3, the output is "1", and when the relative fluorescence intensity is less than 0.3, the output is "0". When there is no input, the molecular beacon is in a closed state, and the fluorescence of the entire computing system is also at a low value. The relative fluorescence intensity is less than 0.3, and the output is recorded as "0". Since miR-155 can trigger molecular beacon (MB2) and hairpin probe (H 2 ), the molecular beacon is turne...

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Abstract

The invention belongs to the technical field of optical molecular logic gates of analytical chemistry and relates to establishment of a reconfigurable, enzyme-free and high-sensitivity miRNA (micro ribonucleic acid) ternary intelligent detection method. The molecular logic gate is constructed based on the magnetic nanospheres and corresponding probe DNA; a fluorescence intensity relative value ofcarboxyfluorescein (FAM) marked on the DNA is used as judgment evidence; when the relative fluorescence intensity is greater than 0.3, the output is 1; when the relative fluorescence intensity is lessthan 0.3, the output is 0. A molecular logic gate system comprises a plurality of cascaded OR logic gates and INHIBIT logic gates, which can be used for detecting whether three different miRNAs (including miR-21, miR-155 and miR Let-7a) exist in an actual sample or not and an existing combination of the three miRNAs in the actual sample. A logic detection system is combined with an assembling technology of target catalysis hairpin DNAs, so that the sensitivity of multi-target analysis is improved; the logic detection system has potential application value on diagnosis of tumor markers.

Description

technical field [0001] The invention belongs to the technical field of optical molecular logic gates of analytical chemistry, and relates to the establishment of a resettable, enzyme-free and high-sensitivity miRNA ternary intelligent detection method. Background technique [0002] MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs found in eukaryotes, which consist of 20-25 ribonucleotides. It can regulate the protein expression of messenger RNA target molecules by combining with specific sequences of messenger RNA (mRNA), and participate in key life activities such as cell development, differentiation, proliferation and death. Studies have shown that more than 60% of protein-coding genes are regulated by miRNA. The stable regulation of miRNA is necessary for all normal physiological processes, and the abnormal expression of miRNA is closely related to the occurrence, metastasis and progression of cancer. In recent years, a large number of miRNAs have been prove...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6816
CPCC12Q1/6816C12Q2563/107C12Q2525/207C12Q2525/301C12Q2563/143C12Q2563/155
Inventor 张思奇
Owner TAIZHOU UNIV
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