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A kind of preparation method and application of three molybdenum tetrasulfide coated gold nanorods

A technology of trimolybdenum tetrasulfide and gold nanorods, which is applied in the field of chemical biosensing and biological detection, can solve the problems of human poisoning, and achieve the effects of low cost, high detection sensitivity, and obvious phenomena

Active Publication Date: 2021-05-04
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once these substances enter the human body, they will cause poisoning and there is no specific drug treatment for animal zearalenone poisoning

Method used

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  • A kind of preparation method and application of three molybdenum tetrasulfide coated gold nanorods
  • A kind of preparation method and application of three molybdenum tetrasulfide coated gold nanorods
  • A kind of preparation method and application of three molybdenum tetrasulfide coated gold nanorods

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Experimental program
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Effect test

Embodiment 1

[0033] Preparation of gold nanorods coated with molybdenum tetrasulfide

[0034] Take 5mL gold nanorods, first add 0.1mmol / L Na 2 S 2 o 3 20μL, then add 0.1mmol / L Mocl 540μL, react for 6 hours, centrifuge at 10000r / min for 10min, and disperse with secondary water to obtain molybdenum tetrasulfide-coated gold nanorods, the shape of which is as follows: figure 1 As shown, the morphology of gold nanorods is completely preserved and a shell-like structure is formed on the surface of gold rods, and the longitudinal plasmon absorption peak of the composite is blue-shifted compared with that of gold nanorods, as shown in figure 2 As shown, this is because the action of molybdenum tetrasulfide and the gold rod changes the refractive index of the surface of the gold rod. image 3 The powder crystal diffraction results show that molybdenum tetrasulfide is coated on the surface of gold nanorods in the form of crystals. Figure 4 The results show that molybdenum tetrasulfide-coated...

Embodiment 2

[0036] Molybdenum tetrasulfide-coated gold nanorods with peroxidase-mimicking properties for the detection of zearalenone:

[0037] First, add 300 μL trimolybdenum tetrasulfide-coated gold nanorods to the centrifuge tube, and then add 500 μL 1.0×10 -13 The DNA aptamer ZEN-aptamer of μmol / L zearalenone was connected to ZEN-aptamer, washed three times with 0.01mol / L PBS, and then added zearalenone solutions of different concentrations, and reacted for 20 minutes with After rinsing with double distilled water for three times, a hydrogen peroxide solution with a concentration of 50 mmol / L was added to react for 30 minutes, and then a TMB solution with a concentration of 5 mmol / L was added. After 30 minutes, its color changes like Image 6 As shown in the figure, from left to right, 0 and 10 are added in order. -7 , 10 -6 , 10 -5 , 10 -4 , 10 -3 , 10 -2 mmol / L of zearalenone, it can be seen from the figure that the higher the concentration of zearalenone, the lighter the col...

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Abstract

The invention discloses a preparation method and application based on molybdenum tetrasulfide-coated gold nanorods. The preparation method is as follows: reacting gold nanorods with sodium thiosulfate solution and molybdenum pentachloride solution for 4 to 8 hours to obtain molybdenum tetrasulfide-coated gold nanorods with peroxide mimetic enzyme activity. The obtained molybdenum tetrasulfide-coated gold nanorod mimic enzyme was used to detect the content of mycotoxin zearalenone. The present invention utilizes the characteristic of catalytically decomposing hydrogen peroxide by molybdenum tetrasulfide-coated gold nanorods, and then according to the specific combination of antigen and antibody, zearalenone and zearalenone are modified by molybdenum tetrasulfide-coated gold nanorods. Enone aptamer-specific action, and then achieve the purpose of detecting zearalenone; the present invention uses molybdenum tetrasulfide coated gold nanorods to detect zearalenone, which has high sensitivity, obvious phenomenon, simple operation and effective Accurately determine the concentration of zearalenone in crops.

Description

technical field [0001] The invention belongs to the technical field of chemical biosensing and biological detection, and in particular relates to a preparation method and application of gold nanorods coated with molybdenum tetrasulfide. Background technique [0002] Enzymes are indispensable to life activities. Many reactions in the human body require the catalysis of enzymes. It can reduce the activation energy of chemical reactions, speed up the rate of chemical reactions, and control the degree of chemical reactions. Natural enzymes are proteins, nucleic acids or their complexes that are produced in vivo and have properties such as high efficiency, specificity, diversity, and ability to react specifically with substrates. They are widely used in food, industry, agriculture, and medicine. Wide range of applications. However, natural enzymes also have many shortcomings. For example, the sensitivity of external environmental conditions (strong acid, strong alkali, high tem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F1/02B22F9/24G01N21/31B82Y40/00
CPCG01N21/31B82Y40/00B22F9/24B22F1/16B22F1/054
Inventor 黄昊文花欣怡汪志芳阳秀梅
Owner HUNAN UNIV OF SCI & TECH
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