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A carbon-based bio-organic fertilizer for improving degraded vegetable soil and its application

A bio-organic fertilizer and vegetable field technology, applied in the field of carbon-based bio-organic fertilizers, can solve the problems of inability to deeply improve the soil biological properties of degraded vegetable fields, unclear soil improvement effects, and single function of carbon-based organic fertilizers. The effect of crop growth, improvement of disease resistance and stress resistance, and the effect of simple field application methods

Active Publication Date: 2021-11-05
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these carbon-based organic fertilizers have a single function and cannot deeply improve the soil biological properties of degraded vegetables, which has limitations.
[0006] CN106495869A discloses a straw charcoal-based bio-organic fertilizer and its preparation method. The patent application uses compost, straw charcoal for adsorbing functional microbial agents, zeolite powder bentonite, and binder, but does not clarify its improvement effect on soil
[0007] Aiming at the current problem of soil degradation in vegetable fields mainly due to nutrient imbalance, poor biological properties and many diseases, it is necessary to develop carbon-based bioorganic fertilizers that can partially replace chemical fertilizers, increase soil organic matter content, improve soil microbial flora, and promote growth and disease prevention. Fertilizer has become a technical problem to be solved urgently in this field

Method used

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  • A carbon-based bio-organic fertilizer for improving degraded vegetable soil and its application
  • A carbon-based bio-organic fertilizer for improving degraded vegetable soil and its application
  • A carbon-based bio-organic fertilizer for improving degraded vegetable soil and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Bacillus amyloliquefaciens BS211 potted disease prevention effect

[0043] Inoculate Bacillus amyloliquefaciens BS211 on a beef extract peptone agar plate and culture at 32°C for 2 days; then pick a single colony of BS211 strain on the plate and transfer it to beef extract peptone liquid medium with a volume of 200m1 / 500m1 , the rotating speed of the shaking table was 160rpm, and the shaking table was cultured at 32°C for 2 days to obtain the BS211 bacterial liquid. At this time, the concentration of the bacterial liquid was counted as 6.3×10 8 CFU / mL.

[0044] Inoculate Bacillus amyloliquefaciens L3 on a beef extract peptone agar plate and culture at 32°C for 2 days; then pick a single colony of L3 strain on the plate and transfer it to beef extract peptone liquid medium with a liquid volume of 200m1 / 500m1 , the rotation speed of the shaking table was 160rpm, and the shaking table was cultured at 32°C for 2 days to obtain the L3 bacterial liquid. At this ...

Embodiment 2

[0047] Embodiment 2 Bacillus amyloliquefaciens L3 sand culture promoting effect

[0048] Get 20 mesh quartz sands, be divided into three groups, add and account for the quartz sands mass percent and be respectively 10% aseptic beef extract peptone culture solution (control group), 10% L3 bacterium liquid (experimental group 1) that embodiment 1 obtains, 10% of the L3 bacterium solution obtained in Example 1+10% of the BS211 bacterium solution obtained in Example 2 (experimental group 2), sow cucumber seeds again, and the real scene of sand culture after 7 days of sowing is as follows figure 2 shown.

[0049] The germination rate of the control group was 71.8%, and the average fresh weight was 2.81g / tree. The germination rate of experimental group 1 was 91.3%, and the average fresh weight was 3.92g / tree. The germination rate of experimental group 2 was 96.9%, and the average fresh weight was 4.52g / tree. It can be seen that the L3 bacteria can significantly promote the growt...

Embodiment 3

[0050] Embodiment 3 is rich in the organic carrier of BS211 and L3 bacillus amyloliquefaciens

[0051] a) Inoculate Bacillus amyloliquefaciens BS211 on a beef extract peptone agar plate, and culture at 32°C for 2 days; then transfer a single colony of BS211 strain picked on the plate to beef extract peptone liquid medium, and the liquid volume is 400m1 / 1000m1, cultured on a shaking table at 32°C for 2 days, the rotating speed of the shaking table was 160rpm; then three parts of 400m1 culture solution were transferred to a 10L seed tank, the inoculum size was 12% (volume ratio), and cultivated at 32°C for 1 day, with an aeration rate of 0.8 L / L min; finally, the seed liquid was transferred to a 200 L fermenter, and cultured at 32°C for 2 days, with an aeration rate of 0.6 L / L min. The biomass of the BS211 bacterial solution out of the tank is 8.2×10 8 CFU / mL, sporulation rate was 94.7%.

[0052] b) Inoculate Bacillus amyloliquefaciens L3 on a beef extract peptone agar plate,...

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Abstract

The invention discloses a carbon-based bio-organic fertilizer for improving the soil of degraded vegetable fields and an application thereof. In the carbon-based bio-organic fertilizer, by mass percentage, 30-40% of straw biochar and 5-15% of potassium fulvic acid , amino acid powder 5-10%, and the balance is an organic carrier rich in BS211 and L3 Bacillus amyloliquefaciens; the carbon-based bio-organic fertilizer of the present invention has obvious improvement effect on degraded vegetable soil, can effectively replace chemical fertilizers, and increase soil organic matter content, improve soil microbial community structure, reduce vegetable soil-borne diseases, promote crop growth, and increase production and income.

Description

technical field [0001] The invention relates to the field of development and application of bio-organic fertilizers, in particular to a carbon-based bio-organic fertilizer for improving the soil of degraded vegetable fields. Background technique [0002] The area of ​​vegetables in China has reached about 340 million mu, the output of vegetables has reached 820 million tons, and the output value has exceeded 2 trillion yuan. Compared with food crops, vegetables have the characteristics of high economic benefits, high multiple cropping index and high planting density. [0003] Improper use of agricultural inputs such as chemical fertilizers, pesticides, plastic sheeting, and livestock and poultry manure has seriously degraded the soil in vegetable fields. Moreover, regional industrialization has led to long-term continuous cropping of vegetables, further deteriorating the soil quality of vegetable fields. At present, the soil of long-term vegetable fields, especially the so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/60C05G3/80C05G3/40C05G3/00
CPCC05C11/00C05F11/08C05G3/00C05G3/80C05D9/00C05F5/006C05F11/02C05F5/00
Inventor 王光飞马艳郭德杰宋修超徐烨红艾玉春
Owner JIANGSU ACAD OF AGRI SCI
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