Detection method of cerebral arterial thrombosis miRNA marker

Abstract

The invention belongs to the technical field of biomedical science, and discloses a detection method of a cerebral arterial thrombosis miRNA marker. According to the miRNA market, miR-622 and miR-221are jointly used as the marker, mature miR-622 and mature miR-221 are selected as the cerebral arterial thrombosis miRNA marker, and the relevance of the miR-622 with the cerebral arterial thrombosisand the relevance of the miR-221 with the cerebral arterial thrombosis are separately determined; detecting the expression level of the miR-622 and the miR-221 in periphery whole blood through a high-flux sequencing technique; and preparing cerebral arterial thrombosis products namely a detection chip and a detection reagent kit. The invention breaks through the detection method for single markers, detection is guaranteed twice, the expression free level is inapparent, the accuracy of the detection results is increased, and valuable treatment time is provided for patients.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for detecting miRNA markers of ischemic stroke. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Ischemic stroke is a general term for brain tissue necrosis caused by stenosis or occlusion of blood supply arteries (carotid artery and vertebral artery) and insufficient blood supply to the brain. There are four types of cerebral ischemia: transient ischemic attack (TIA); reversible neurological impairment (RIND); progressive stroke (SIE); and complete stroke (CS). TIA has no cerebral infarction, but RIND, SIE and CS have different degrees of cerebral infarction. Cerebral ischemia can be divided into localized cerebral ischemia and diffuse cerebral ischemia according to the scope of ischemia. The causes of focal cerebral ischemia are: middle cerebral artery embolism; extracranial in...

AI Technical Summary

Problems solved by technology

[0007] (1) Traditional detection methods such as CT and MRI scans, cerebrovascular examination, and transcranial Doppler examination are limited by the onset, onset time, and onset degree, and cannot quickly and accurately diagnose the disease;

[0008] (2) The existing miRNA markers used in ischemic stroke are only a single miRNA marker, which cannot achieve the double insurance of detection. If the expression level of this marker is not obvious, the detection rate should be reduced. Accuracy of results, thereby affecting the timeliness of patient treatment

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