Micro magnet cell capturing device and manufacturing method thereof

A capture device and manufacturing method technology, applied in the field of biotechnology applications, can solve the problems of affecting the structural integrity and fluorescent signal of circulating tumor cells, interfering with cell imaging and identification, and poor cell capture effect, achieving operability and reliability. Good reproducibility, reduced cell loss, and reduced cell aggregation

Inactive Publication Date: 2019-04-12
NINGBO MEIJING MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the above method still has the following defects in the process of use: on the one hand, when the blood flows through the microfluidic channel, it is limited by the low magnetic field gradient, and the cell capture effect is not strong, which may lead to cell loss; on the other hand, Low effective magnetic trap density can lead to cell aggregation at the front of the microfluidic channel, which can affect the structural integrity and fluorescent signal of circulating tumor cells, interfering with cell imaging and identification

Method used

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  • Micro magnet cell capturing device and manufacturing method thereof
  • Micro magnet cell capturing device and manufacturing method thereof
  • Micro magnet cell capturing device and manufacturing method thereof

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Embodiment 1

[0040]As shown in the figure, a micro-magnet cell capture device of the present invention includes a microfluidic channel layer 1, a cell capture chip 2 and a permanent magnet 3 that are arranged in close proximity from top to bottom. The bottom of the microfluidic channel layer 1 is provided with A groove, a microfluidic channel 4 is formed between the groove and the upper surface of the cell capture chip 2, and the position of the permanent magnet 3 corresponds to the position of the microfluidic channel 4. The cell capture chip 2 includes a substrate 5, and the upper surface of the substrate 5 is connected to the The corresponding position of the microfluidic channel 4 forms a cell capture area 6 , which is preferably hexagonal, and a plurality of evenly distributed micromagnets 7 are arranged in the cell capture area 6 .

[0041] In this embodiment, the microfluidic channel 4 is a flat cavity with a height of 500 μm, thereby increasing the contact area between the sample an...

Embodiment 2

[0047] As shown in the figure, a micro-magnet cell capture device, the rest of the structure is the same as the first embodiment, the difference is that: in this embodiment, the permanent magnet 3 is composed of a plurality of permanent magnet units 16 with the same shape and size, a plurality of The permanent magnet units 16 are spliced ​​into a plate shape on a two-dimensional plane. In the present embodiment, the permanent magnet 3 is a 6 × 6 array composed of 36 permanent magnet units 16, wherein the permanent magnet unit 16 is a cube magnet whose side length is 1 / 8 inch for use as NB001-N48, Plano, TX piece. The permanent magnet is an important part of the immunomagnetic bead method to capture cells. Compared with the traditional monolithic permanent magnet structure, the magnetic field of the permanent magnet structure of the present invention is alternately polarized, which is displayed as a dispersed potential well on the microchannel, making the magnetic field distrib...

Embodiment 3

[0053] A method for manufacturing a micro-magnet cell capture device of the present invention, comprising:

[0054] arranging a plurality of magnetic nanoparticles in the cell capture area 6 on the upper surface of the substrate 5 according to a set structure, and forming a plurality of evenly distributed micro-magnets 7 to obtain a cell capture chip 2;

[0055] Cover the upper surface of the cell capture chip 2 with a layer of SiO with uniform thickness 2 Membrane 15, so that the micro-magnet 7 is not affected by the flow of liquid in the micro-fluidic channel 4;

[0056] Prepare the microfluidic channel layer 1 to form grooves at the bottom;

[0057] The microfluidic channel layer 1, the cell capture chip 2 and the permanent magnet 3 are closely attached in sequence, so that the groove at the bottom of the microfluidic channel layer 1 corresponds to the position of the cell capture area 6 on the upper surface of the cell capture chip 2, so that the position between them A ...

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Abstract

The invention discloses a micro magnet cell capturing device and a manufacturing method thereof. The micro magnet cell capturing device comprises a micro fluid channel layer, a cell capturing chip anda permanent magnet, which are sequentially attached from top to bottom, wherein a groove is formed in the bottom of the micro fluid channel layer; and a micro fluid channel is formed between the groove and the upper surface of the cell capturing chip. The micro magnet cell capturing device is characterized in that the cell capturing chip comprises a base; the upper surface of the base and a corresponding position of the micro fluid channel form a cell trapping region; and a plurality of micro magnets which are uniformly distributed are arranged in the cell trapping region. The micro magnet cell capturing device disclosed by the invention has the advantages that the cell capturing rate can be increased, and cell damage is reduced; and the gathering of cells in the front section of the micro fluid channel can be reduced, and subsequent analysis and research of the cells is facilitated.

Description

technical field [0001] The invention relates to the application field of biotechnology, in particular to a micro-magnet cell capture device and a manufacturing method thereof. Background technique [0002] Circulating tumor cells (CTCs, circulating tumor cells) are a general term for all kinds of tumor cells that exist in peripheral blood. They are released from tumor lesions, fall off from solid tumor lesions (primary lesions, metastases) and enter the blood circulation. The number of rare cells is an important indicator of disease progression and metastasis in cancer patients. The detection of circulating tumor cells is helpful for the diagnosis of patients with early metastatic tumors, monitoring the recurrence and metastasis of postoperative patients, evaluating the sensitivity of antitumor drugs and the prognosis of patients, and selecting individualized treatment strategies. However, the number of circulating tumor cells in the blood is very rare, with an average of 1...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/42
CPCC12M23/16C12M35/06
Inventor 张晓晶沈挺
Owner NINGBO MEIJING MEDICAL TECH
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