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Vitreous substitute material suitable for proliferative vitreoretinopathy and preparation method thereof

A vitreous retinal and vitreous body technology, applied in prosthetics, pharmaceutical formulations, tissue regeneration, etc., can solve the problems of accelerating hydrogel degradation and absorption, degradation of hydrogel mechanical properties, and reducing intraocular filling time, etc., to achieve long-term existence, Pain-relieving, bio-friendly effect

Active Publication Date: 2021-07-06
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, this kind of hydrogel is mainly obtained through in vitro cross-linking, and then injected into the vitreous cavity through an injection needle, which has the following defects: the hydrogel structure will be destroyed during the injection process to form polymer fragments, which will lead to water loss. The mechanical properties of the gel decrease; and the formation of polymer fragments will accelerate the degradation and absorption of the hydrogel, reducing the filling time in the eye
On the other hand, current research shows that only hydrogel can effectively combine drugs and improve the therapeutic effect of PVR surgery, while inert gas, silicone oil, heavy silicone oil, perfluorocarbon liquid (heavy water), etc. cannot combine with drugs
However, the long-term safety and effectiveness of such hydrogels for intraocular filling still need to be further improved to better treat patients
[0009] The current study found that the PVA hydrogel with properties similar to the natural vitreous body was obtained through in vitro cross-linking, and it was injected into the body for half a year. The experimental observation found that: 3% PVA hydrogel has good optical and physical properties, retinal support Function and biocompatibility; however, the structural damage of the hydrogel caused by the injection process and the degradation and absorption of the hydrogel after long-term filling cannot be avoided

Method used

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  • Vitreous substitute material suitable for proliferative vitreoretinopathy and preparation method thereof
  • Vitreous substitute material suitable for proliferative vitreoretinopathy and preparation method thereof
  • Vitreous substitute material suitable for proliferative vitreoretinopathy and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] (1) Heat 7% PVA aqueous solution to 80°C to dissolve it completely, take β-sodium glycerophosphate and dissolve it in acetic acid aqueous solution to make 2.5% sodium β-glycerophosphate solution, filter and remove impurities after the dissolution is complete, and then follow 1: Mix PVA aqueous solution and β-sodium glycerophosphate solution at a ratio of 1.5, slowly cool down to room temperature and add 2% calcium chloride solution dropwise until the volume becomes 1.5 times the original volume, so that cross-linking reaction occurs, using dihydrogen phosphate Adjust the pH value of the sodium saturated solution to 7.21, and dialyze in distilled water for 48 hours after forming a gel to remove the residue of non-crosslinked PVA to obtain a PVA hydrogel;

[0059] (2) Mix 5-fluorouracil and PLGA in a ratio of 1:10 and dissolve in dichloromethane, then slowly add it dropwise to liquid paraffin containing Span 80, heat at 60°C, stir and emulsify at a speed of 1800rpm 1h, ob...

Embodiment 2

[0064] (1) Heat 1% PVA aqueous solution to 80°C to make it completely dissolve, take chitosan and dissolve it in acetic acid aqueous solution to make 2.5% chitosan solution, filter and remove impurities after dissolving completely, and then mix PVA aqueous solution and chitosan solution were mixed, slowly cooled to room temperature and 2% calcium chloride solution was added dropwise until the volume became 1.5 times of the original volume, so that cross-linking reaction occurred, and the pH value was adjusted using disodium hydrogen phosphate saturated solution. Regulate 7.08, dialyze in distilled water for 48 hours after forming gel, to remove the residue of non-crosslinked PVA, make PVA hydrogel;

[0065] (2) Mix 5-fluorouracil and PLGA at a ratio of 1:9 and dissolve in acetone, then slowly add it dropwise to liquid paraffin containing Span 80, heat at a constant temperature at 55°C, stir and emulsify at 2000rpm for 1h, get lotion;

[0066] (3) Add a large amount of sherwoo...

Embodiment 3

[0070] (1) Heat 3% PVA aqueous solution to 80°C to make it completely dissolve, take chitosan and dissolve it in acetic acid aqueous solution to make 2% chitosan solution, filter and remove impurities after dissolving completely, and then dissolve the chitosan according to the ratio of 1:1 PVA aqueous solution and chitosan solution were mixed, slowly cooled to room temperature and 2% calcium chloride solution was added dropwise until the volume became 1.5 times of the original volume, so that cross-linking reaction occurred, and the pH value was adjusted using disodium hydrogen phosphate saturated solution. Regulate 7.14, after forming gel, dialyze in distilled water for 48 hours, to remove the residue of non-crosslinked PVA, make PVA hydrogel;

[0071] (2) Mix 5-fluorouracil and PLGA at a ratio of 1:9 and dissolve in acetonitrile / DMF composite solvent, then slowly add it dropwise to liquid paraffin containing Span 80, heat at a constant temperature of 50°C at a speed of 1500rp...

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Abstract

The invention discloses a vitreous substitute material suitable for proliferative vitreoretinopathy, which is prepared by the following steps: (1) heating a PVA aqueous solution, adding a crosslinking agent to generate a crosslinking reaction, adjusting the pH value to neutral, Prepare PVA hydrogel; (2) Dissolve 5-fluorouracil and PLGA in an organic solvent, then slowly add it dropwise to liquid paraffin containing an emulsifier, heat and stir at a constant temperature to emulsify to obtain an emulsion; (3) Add to step (2 ) Add petroleum ether to the emulsion until all the emulsion forms a precipitate, and centrifuge to obtain microspheres; (4) use petroleum ether to centrifugally wash the microspheres, and obtain 5-fluorouracil microspheres after drying; (5) coagulate the PVA Glue and 5‑fluorouracil microspheres were mixed and stirred under heating to obtain a vitreous substitute material. The material has properties close to those of the natural vitreous, providing an ideal treatment for patients with proliferative vitreoretinopathy.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a vitreous substitute material suitable for proliferative vitreoretinopathy and a preparation method thereof. Background technique [0002] Proliferative vitreoretinopathy (PVR) is a common blinding eye disease that often occurs in patients with ocular trauma, rhegmatogenous retinal detachment and severe diabetic retinopathy. [0003] At present, vitrectomy is the main method for the treatment of PVR. After the natural vitreous is removed during the operation, a suitable artificial vitreous substitute must be selected to fill the vitreous cavity to repair eye damage, maintain the support of the retina, and attach the retinal pigment epithelium to the neuroepithelium for reconstruction. Improve visual function and prevent eyeball atrophy. [0004] The artificial vitreous body is the most interesting and challenging research field in the field of ophthalmology. After vitrectomy ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/16A61L27/50A61L27/52A61L27/54A61L27/58
CPCA61L27/16A61L27/50A61L27/52A61L27/54A61L27/58A61L2300/622A61L2300/802A61L2400/06A61L2430/16
Inventor 冯松福余震陆晓和崔桓
Owner ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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