Method for breeding rape self-incompatible hybrid seeds under assistance of S haplotype molecular markers

Abstract

The invention belongs to the technical field of rape molecular breeding, and discloses a method for breeding rape self-incompatible hybrid seeds under assistance of S haplotype molecular markers. Themethod comprises the following steps: obtaining F1 seeds, detecting S haplotype of a selfing line, obtaining self-incompatible two-line and three-line hybrid seeds, and arranging a variety screening test by the obtained two-line hybrid seeds and three-line hybrid seeds. The S haplotype of F1 is identified by double PCR markers, the technical defect that no marker is generated due to accidental factors in the experimental process is overcome, and the S haplotype of the selfing line and recovery or retention of the S haplotype can be accurately judged; fussiness of fertility identification and blindness of combination configuration in the breeding process of rape self-incompatible hybrids are overcome with the molecular marker technology, the breeding workload is greatly reduced, the breeding cycle is shortened, breeding efficiency is improved, and rapid cultivation of the cabbage type rape self-incompatible hybrids is promoted.

Description

technical field [0001] The invention belongs to the technical field of rapeseed molecular breeding, in particular to a method for assisted breeding of rapeseed self-incompatibility hybrids by S haplotype molecular markers. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Self-incompatibility (Self-incompatibility, SI) is a ubiquitous natural phenomenon in the plant world, where the stamens and stamens develop normally but self-crossing or sibling crossing produces few or no seeds. It is an important way to utilize plant heterosis. Natural cabbage and cabbage are self-incompatible, and self-incompatibility has been widely used in breeding hybrids of cabbage and cabbage, and the planting of hybrids has significantly improved the yield of cabbage and cabbage. Brassica napus (or called rape) (AACC, 2n=38) is naturally crossed and doubled from Chinese cabbage (AA, 2n=20) and Brassica oleracea (CC, 2n=18), ...

AI Technical Summary

Problems solved by technology

This method can identify the compatibility of plants at the flowering stage, but it is difficult to apply it to field breeding: first, it cannot accurately judge the compatibility / incompatibility, and some plants do not elongate their pollen tubes at the early flowering stage and show strong self-indulgence. Cross-incompatibility, self-incompatibility disappears in late flowering stage, and self-fertilization can still set seeds normally; second, it needs to be carried out in the flowering stage, and the detection time is extremely short, so it is impossible to identify the phenotype of a large number of plants

[0015] The present invention attempts to identify plant compatibility / incompatibility by using molecular marker technology, but does not overcome the technical defect of not producing markers due to accidental factors in the experimental process

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