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Test card for liver function and preparation method thereof

A test card and liver function technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of high testing cost, difficult popularization, poor mobility, etc., and achieve the effect of simple operation

Active Publication Date: 2019-04-05
杭州联晟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The kit is mainly used in combination with large-scale biochemical analyzers, the detection cost is high, professional and technical personnel are required to operate, the mobility is poor, and it is difficult to popularize

Method used

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  • Test card for liver function and preparation method thereof
  • Test card for liver function and preparation method thereof
  • Test card for liver function and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0045] A method for preparing a liver function test card, comprising the steps of:

[0046] Prepare alanine aminotransferase reaction liquid: 10-40KU pyruvate oxidase, 1-20KU peroxidase, 1-20KU ascorbic acid, 0.1-2g L-alanine, 1-4g α-ketoglutarate, 0.1-3g coke Thiamine phosphate, 0.1-2g flavin adenine dinucleotide, 0.5-4g film former, 2-20g stabilizer, 0.1-5g surfactant, 0.1-10g developer, 0.1-1M buffer Stir for one hour and set aside;

[0047] Prepare total bilirubin reaction solution: 1-50g basic sodium tartrate, 0.1-5g surfactant, 0.1-1M buffer solution are stirred for one hour and then used;

[0048] Prepare aspartate transaminase reaction solution: the aspartate transaminase reaction solution includes: 5-50KU pyruvate oxidase, 5-40KU oxaloacetate decarboxylase, 1-50KU peroxidase, 5-40KU ascorbic acid, 0.1-5g L-asparagus Sodium phosphate, 0.1-5g α-ketoglutarate, 0.1-5g thiamine pyrophosphate, 0.5-4g film former, 2-20g stabilizer, 0.1-5g surfactant, 0.1-10g developer, 0.1...

Embodiment 1

[0057] The preparation method of finished product 1 comprises the following steps:

[0058] Step 1: prepare alanine aminotransferase reaction solution: 10KU pyruvate oxidase, 7KU peroxidase, 5KU ascorbic acid 0.3gL-alanine, 1gα-ketoglutarate, 0.5g thiamine pyrophosphate, 0.5g yellow Adenine dinucleotide, 20ml 1.5% polyvinyl alcohol, 1g bovine serum albumin, 1g trevallyte, 0.1gtritonX-100, 0.2gN-ethyl-N-(2-hydroxy-3-sulfopropane base)-3'5-dimethoxyaniline sodium salt, 1g of 4-aminoantipyrine was added to 1L of 0.2M TRIS buffer with a pH value of 6.8 and stirred for one hour before use;

[0059] Step 2: prepare total bilirubin reaction liquid: join 5g basic sodium tartrate and 0.1gEmulgen B66 in the phosphate buffer solution that 1L0.2MpH value is 7.2, stir one hour and stand-by;

[0060] Step 3: Prepare aspartate transaminase reaction solution: 10KU pyruvate oxidase, 5KU oxaloacetate decarboxylase, 5KU peroxidase, 5KU ascorbic acid 0.2g L-sodium aspartate, 0.5g α-ketoglutarate...

Embodiment 2

[0067] The preparation method of finished product 2 comprises the following steps:

[0068] Step 1: Prepare alanine aminotransferase reaction solution: 25KU pyruvate oxidase, 10KU peroxidase, 10KU ascorbic acid, 1gL-alanine, 2gα-ketoglutarate, 1g thiamine pyrophosphate, 1g flavin adenine Dinucleotide, 100ml 1.5% polyvinyl alcohol, 10g bovine serum albumin, 2g trevallyte, 1gtritonX-100, 2gN-ethyl-N-(2-hydroxy-3-sulfopropyl)-3' Add 5-dimethoxyaniline sodium salt, 3g 4-aminoantipyrine into 1L 0.1M TRIS buffer with a pH value of 6.8 and stir for one hour before use;

[0069]Step 2: prepare total bilirubin reaction liquid: join 20g basic sodium tartrate and 0.5gEmulgen B66 in the phosphate buffer solution that 1L0.1MpH value is 7.2, stir for one hour and stand-by;

[0070] Step 3: prepare aspartate transaminase reaction liquid: with 25KU pyruvate oxidase, 15KU oxaloacetate decarboxylase, 20KU peroxidase, 15KU ascorbic acid 1gL-sodium aspartate, 2g α-ketoglutarate, 1g sulfur pyroph...

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Abstract

The invention discloses a test card for the liver function and a preparation method thereof. The test card comprises an upper shell, a lower shell, a sample adding hole formed in the upper shell, an alanine transaminase detecting unit, a total bilirubin detecting unit and a glutamic oxalacetic transaminase detecting unit, wherein the alanine transaminase detecting unit, the total bilirubin detecting unit and the glutamic oxalacetic transaminase detecting unit are disposed between the upper shell and the lower shell. The alanine transaminase detecting unit, a total bilirubin detecting unit anda glutamic oxalacetic transaminase detecting unit comprise a bottom plate disposed at the upper end of the lower shell, lower shell detecting holes formed in the lower shell, bottom plate detecting holes formed in the bottom plate, a diffusion film disposed at the lower end of the upper shell and connected to the bottom plate, a whole blood separation layer disposed under the diffusion film and areaction layer disposed between the whole blood separation layer and the bottom plate detecting holes. The test card is matched with a dry biochemical analyzer for use, the operation is simple, the content of alanine transaminase, total bilirubin and glutamic oxalacetic transaminase can be detected rapidly and simultaneously without the need of a professional, and the detecting results are well matched with hospital detecting results.

Description

technical field [0001] The invention relates to the detection field of dry chemistry, in particular to a liver function test card and a preparation method thereof. Background technique [0002] Viral hepatitis exists widely in the world and seriously threatens human health. It is particularly important for the detection of liver function. There are four main indicators for liver function detection: transaminase, bilirubin, albumin and liver fiber. Now hospitals basically use Using biochemistry to check, the operation is complicated, the fee is expensive, and it needs professionals to operate. [0003] For liver function tests, only transaminases are the only items tested by dry chemical methods on the market, such as Aikang biological ALT and AST test strips, the corresponding patent number is 201010542684.X, and the ALT / GPT test strips of Nanjing Dashu, corresponding The patent number is CN 206146942 U, etc. Although there are several related products in China, such as the...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N33/573
CPCG01N33/50G01N33/573
Inventor 陈军刚
Owner 杭州联晟生物科技有限公司
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