Single-index microfluidic chip, and production method and application method thereof

Abstract

The invention discloses a single-index microfluidic chip, and a production method and an application method thereof. The single-index microfluidic chip comprises a chip body. A sampling cavity, a quantitative reaction cavity and a waste liquid cavity are formed in the chip body; a first backflow prevention device and a second backflow prevention device are arranged at the front end and the rear end of the quantitative reaction cavity correspondingly; at least one of the first backflow prevention device and the second backflow prevention device is a rubber plug anti-backflow device that comprises a rubber plug, a fluid input pipe capable of raising the fluid delivery height and a fluid output pipe capable of lowering the liquid delivery height. The front end and the rear end of the quantitative reaction cavity are provided with the backflow prevention devices, and accordingly, quantification of fluids in the quantitative reaction cavity can be guaranteed; during antibody / antigen coating, antibody / antigen coating solvents can be injected into the fluid input pipe / fluid output pipe of the rubber plug anti-backflow device, and then are subjected to incubation, washing, packaging, and vacuumizing by a vacuum drying oven before being provided with a rubber plug, so that the single-index microfluidic chip is suitable for mass production. Basically, the quality of coated antibodies / antigens is not affected by the packaging time and the packaging process.

Description

technical field [0001] The invention relates to a microfluidic chip; [0002] The present invention also relates to a production method and a use method of the aforementioned microfluidic chip. Background technique [0003] As a stable and practical technology, immunological lateral flow diagnostic technology is suitable for use in various point-of-care tests (POCT) or on-site. In the immunochromatographic reaction system, due to system reasons, the CV is large and accurate quantification cannot be achieved. The immunodiagnostic method based on microfluidic technology can effectively avoid the above problems. [0004] There are two types of microfluidics: passive and active. Among them: Passive microfluidics requires capillary force to achieve liquid-forward lateral flow. However, due to the different viscosities of different samples, especially whole blood samples, the liquid flow rate cannot be uniform. Active microfluidics can effectively avoid the above problems, an...

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Problems solved by technology

[0006] 1. When the coated antibody and labeled antibody are pre-embedded in the quantitative reaction chamber, when they react with the filtered plasma introduced through the sample injection chamber during detection, non-specific reactions are prone to occur, thereby affecting the detection accuracy;

[0007] 2. When using the sampling cavity described in the above documents for whole blood filtration, due to the air holes in the sampling part of the sampling cavity, gas pressure will be generated on the filter paper, resulting in poor sample flow, which is not as expected by the applicant. Contrary to the original intention that the configuration of vent holes will improve the poor blood flow;

[0008] 3. In the production process, the layered microfluidic chips currently on the market are generally coated with antibodies first, and then the chips of each layer are bonded and assembled as soon as possible, otherwise the quality of the coated antibodies may be affected, and the bonding process It may also affect the quality of the coated antibody, resulting in inaccurate test results

[0009] 4. Usually, the test results of the microfluidic chip are related to the external testing environment; in addition, although we will indicate the service life of the microfluidic chip itself, the actual storage environment is improper, which will affect the detection accuracy of the chip; therefore, Chip quality control is required to monitor the effectiveness of the chip itself

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