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Microbial preparation, aquatic feed and aquaculture method

A technology of microbial preparations and probiotics, applied in the fields of aquafeed, aquaculture, and microbial preparations, can solve the problems of destroying beneficial flora, easy disease of farmed animals, drug resistance, etc., to improve growth rate, treat or prevent arctic Bacterial infection, the effect of enhancing food intake

Inactive Publication Date: 2019-04-05
SHANGHAI LYUAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, while antibiotics kill pathogenic bacteria, they also kill other beneficial bacteria in animals at the same time, seriously destroying the original beneficial bacteria in animals, breaking the microbial balance in the body, and constantly being broken. The microbial balance system has a great impact on the body's immunity, making farmed animals more and more prone to disease; secondly, while feeding antibiotics for a long time to remove pathogenic bacteria, it is easy to cause drug resistance problems, and eventually lead to breeding The success rate and antibiotic residues are getting worse

Method used

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  • Microbial preparation, aquatic feed and aquaculture method
  • Microbial preparation, aquatic feed and aquaculture method
  • Microbial preparation, aquatic feed and aquaculture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] preparation of microbial preparations

[0101] 1 Preparation of dry powder of Bdellovibrio bacteriophage

[0102] (1) Preparation of host bacterium concentrate: first pick a single colony of Escherichia coli, inoculate it in a nutrient broth liquid medium (20g / L), and cultivate it for 24h at 150rpm and 30°C to obtain a culture solution; Centrifuge the culture medium under the conditions of 4000rpm, 4°C, and 25min for 25min, collect the precipitate, suspend it with 2.5ml DNB liquid medium for every 80ml of culture medium, and obtain the concentrate of the host bacteria, store it in a refrigerator at 4°C Save it for later use.

[0103] The nutrient broth liquid medium used was 20 g / L nutrient broth, pH 6.8, and was autoclaved at 121° C. for 20 minutes before use.

[0104] (2) Preparation of bacteriophage Bdellovibrio: mix according to the ratio of bacteriophage Bdellovibrio, host bacterium concentrate: the volume ratio of DNB liquid medium is 1:1:50, the condition of co...

Embodiment 2-36

[0120]The microbial preparations of Examples 2-36 are basically the same as those in Example 1, except that the amounts and proportions of Bdellovibrio lyophilized powder, probiotic lyophilized powder, fish meal and soybean meal used are different, as shown in the table 1.

[0121] Table 1

[0122]

[0123]

experiment example 1

[0125] Detect the effect of inhibiting pathogenic bacteria infection of the microbial preparation of embodiment 1-36

[0126] The experiment was carried out in the Penaeus vannamei experimental base in Dongsi Town, Longhai City, Fujian Province, with a total of 120 ponds, each with a water depth of 18 cubic meters and 1 meter. The experimental period is 30 days. The experiment was divided into 36 groups, with 3 repetitions in each group. The number of prawns in each pond was 8000, and the size of prawns was 1cm. The first group is the blank control group, and the control group 1 is Bdellovibrio bacteriophage content is 10 3 pfu / g+ Lactobacillus germanus subsp. bulgaricus 10 4 cfu / g+20%% fishmeal+35% soybean meal group, the control group 2 is Bdellovibrio phage content of 10 10 pfu / g+ Lactobacillus germanus subsp. bulgaricus 10 11 cfu / g+20% fish meal+35% soybean meal group, the control group 3 is Bdellovibrio phage content of 10 4 pfu / g+common feed group. The remaining gr...

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Abstract

The invention discloses a microbial preparation, an aquatic feed and an aquaculture method and relates to the technical field of aquaculture. The microbial preparation comprises bdellovibrio and probiotic. Optimally, the probiotic is selected from any one of lactobacillus bulgaricus, lactobacillus delbrueckii, enterococcus faecalis, lactobacillus casei, lactobacillus acidophilus, rhodopseudomonaspalustris, clostridium butyricum, bacillus laterosporus, bacillus pumilus, bacillus coagulans, bacillus licheniformis, bacillus subtilis and lactobacillus plantarum. The microbial preparation has thecharacteristics of capability of increasing food intake of aquaculture animals, capability of promoting growth rate, survival rate and immunity of organisms, and the like. Besides, the microbial preparation is capable of effectively preventing and treating the problems of diseases caused by pathogenic bacteria, such as vibrio parahaemolyticus, vibrio harveyi, luminous vibrio, vibrio alginolyticus,vibrio campbellii, vibrio fluvialis, vibrio vulnificus, aeromonas, vibrio cholerae, pseudomonas and salmonella.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular, to a microbial preparation, an aquaculture feed and an aquaculture method. Background technique [0002] The use of feed additives containing pharmaceutical ingredients is becoming more and more widely, especially in recent years, the application of antibiotics in aquatic feed. Antibiotics have played a great role in the prevention and treatment of animal diseases, but at the same time, they have also brought great harm to farmed animals and humans. brought certain side effects. First of all, while antibiotics kill pathogenic bacteria, they also kill other beneficial bacteria in animals at the same time, seriously destroying the original beneficial bacteria in animals, breaking the microbial balance in the body, and constantly being broken. The microbial balance system has a great impact on the body's immunity, making farmed animals more and more prone to disease; secondly, wh...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/18A23K10/22A23K10/37A23K10/30A23K20/28A23K40/10A01K61/59
CPCA01K61/59A23K10/18A23K10/22A23K10/30A23K10/37A23K20/28A23K40/10A23K50/80A23V2400/125A23V2400/113A23V2400/225A23V2400/169Y02A40/818Y02A40/81Y02P60/87
Inventor 李家胜吕航
Owner SHANGHAI LYUAO BIOTECH CO LTD
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