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Microfluidic chip, tumor metastasis model based on chip, model construction method and application

A microfluidic chip and tumor metastasis technology, which is applied in the fields of cell biology and tissue engineering, can solve the problems of seldom simulated anti-tumor drug screening, etc.

Inactive Publication Date: 2019-03-26
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many researchers have used microfluidic chip technology to study tumor cell migration and invasion, but there are few studies on the simulation of the whole process of tumor metastasis and the screening of anti-tumor drugs.

Method used

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  • Microfluidic chip, tumor metastasis model based on chip, model construction method and application
  • Microfluidic chip, tumor metastasis model based on chip, model construction method and application
  • Microfluidic chip, tumor metastasis model based on chip, model construction method and application

Examples

Experimental program
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Effect test

Embodiment 2

[0059] Example 2 Microfluidic chip-based tumor metastasis model and its construction method

[0060] Such as Figure 5 As shown, a tumor metastasis model based on a microfluidic chip, the porous membrane in the microfluidic chip is coated with collagen to form a three-dimensional microenvironment; the microfluidic channels of the second chip layer are perfused with vascular endothelial cells to form dense blood vessels in vitro Lumen; Tumor cells are injected into the microfluidic channels of the first chip layer by continuous perfusion.

[0061] The construction method of above-mentioned tumor metastasis model, described construction method comprises the following steps:

[0062] Step 1: Perfusion of collagen within the chip

[0063] Configure collagen with a final concentration of 0.1-10 mg / ml, use a syringe pump to pour collagen from the channel inlet of each layer of the chip into the three-dimensional channel channel by continuous perfusion flow, place the chip in a 37°...

Embodiment 3

[0068] Embodiment 3 Real-time monitoring of the whole process of tumor metastasis

[0069] Using the tumor metastasis model established by the microfluidic chip described in Example 1-2, the cultured vascular endothelial cells (EAhy926) were digested into a single cell suspension, and the cell suspension (0.2*10 6 cells / ml) were injected into the microfluidic channel of the second chip layer by continuous perfusion. After filling the cell suspension, the chip was placed in a 37°C incubator for 2 hours, and the lower surface of the cell culture chamber of the second chip layer Form a dense cell layer; then turn the chip upside down, perfuse the cell suspension twice to form a dense cell layer on the upper surface, and then use a syringe pump to drive the liquid to continue culturing vascular endothelial cells for 3 days to form a dense vascular cavity in vitro. The cultured tumor cells were digested into a single cell suspension, and the cell suspension (0.1*10 6 cells / ml) was...

Embodiment 4

[0070] Example 4 Migration characteristics of different tumor cells

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Abstract

The invention discloses a microfluidic chip, a tumor metastasis model based on the chip, a model construction method and application. The microfluidic chip comprises three layers of chip layers, two layers of porous membranes and two layers of fixed plates, wherein each chip layer is provided with one cell culture chamber with the diameter of 0.1 to 10mm and a micro-fluid channel with the width of0.1 to 10mm and the height of 10 to 500mum, the pore diameter of each porous membrane is 0.1 to 20mum and the porous membranes can be used for realizing cell wall attachment and mutual communication.The construction method of the tumor metastasis model comprises the following steps: 1) filling collagen into the chip by adopting the microfluidic chip and coating the porous membranes; 2) filling vascular endothelial cells into the second layer of the chip and constructing a blood vessel cavity model; and 3) filling tumor cells into the first layer of the chip. The model can be used for researches of different tumor cell metastasis and screening of anti-tumor medicines, and has the characteristics of stability, reliability and high integrated level.

Description

technical field [0001] The invention belongs to methods of cell biology and tissue engineering, in particular to a microfluidic chip, a tumor metastasis model based on the chip, a method for constructing the model, and an application thereof. Background technique [0002] Tumor is one of the diseases that are extremely harmful to human health. According to statistics, 90% of tumor-related deaths are related to tumor metastasis. However, due to the complex physiological environment of humans and animals, people still know little about the metastasis mechanism of tumor cells. It is particularly important to develop an effective real-time monitoring platform for tumor metastasis to fully understand the process and mechanism of tumor cell metastasis and to develop effective anti-metastasis methods. Due to the fundamental species differences between traditional animal models and humans, the development cycle of anti-tumor drugs is long and expensive. However, the existing tradi...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/09C12Q1/02
CPCC12M23/16C12M25/04C12N5/069C12N5/0693C12N2503/02C12N2513/00C12N2533/54G01N33/5011G01N33/5044
Inventor 杜昱光荆柏林李建军王倬
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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