PLC (Primary Liver Cancer) perioperative period liver transplantation patient circRNA differential expression profile map model as well as building method and building system thereof

Abstract

The invention discloses a PLC (Primary Liver Cancer) perioperative period liver transplantation patient circRNA differential expression profile map model as well as building method and building systemthereof. The building method comprises the following steps: setting a disease group and a control group, wherein each group includes a plurality of peripheral blood specimens of different human bodies, which are already separated from the human bodies; extracting RNAs respectively; analyzing circRNA expression profiles in RNA solutions of the disease group and the control group by adopting a circRNA microarray chip to obtain circRNA differential data; verifying the expression situations of circRNA in the disease group and the control group by adopting RT-PCR to obtain comparison data; performing data analysis on the circRNA differential data and the comparison data to build the PLC perioperative period liver transplantation patient circRNA differential expression profile map model. According to the building method, differentially-expressed circRNAs among a patient before liver transplantation, the patient subjected to liver transplantation and a healthy control are compared to screenPLC specifically-expressed circRNAs and circRNAs which are correlated with post-transplantation early liver function recovery, so that new information is provided for the early diagnosis, prognosis and treatment of PLC.

Description

technical field [0001] The invention relates to the field of biomedical technology, in particular to a circular RNA differential expression profile model for PLC perioperative liver transplantation patients and its construction method and construction system. Background technique [0002] Primary liver cancer (Primary Liver Cancer, PLC) is the most common malignant liver tumor in my country. The clinical manifestations of the disease are complex, and it is likely to occur in men aged 40 to 50. It is affected by many factors, such as chemical elements including trace elements. , environmental factors and viral infection, so its pathogenesis is complex and has not been elucidated, which also brings difficulties to the treatment of PLC. At present, liver transplantation is the most effective and thorough method to treat PLC. It removes the entire cancerous liver tissue and replaces it with matching healthy liver tissue, so that the tumor can be removed as much as possible and re...

AI Technical Summary

Problems solved by technology

[0002] Primary liver cancer (Primary Liver Cancer, PLC) is the most common malignant liver tumor in my country. The clinical manifestations of the disease are complex, and it is likely to occur in men aged 40 to 50. It is affected by many factors, such as chemical elements including trace elements. , environmental factors and viral infection, so its pathogenesis is complex and has not been elucidated, which also brings difficulties to the treatment of PLC

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