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Tissue culture and rapid propagation method for aristolochia debilis

A technology of tissue culture and mountain grass fruit, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low seed setting rate, difficult to obtain seedlings, etc., and achieve the effect of meeting the needs of large-scale production

Active Publication Date: 2019-03-22
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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  • Abstract
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  • Claims
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Problems solved by technology

[0003] In order to achieve the above object, the present invention provides a tissue culture rapid propagation method of Limegrass, which solves the problems in the prior art that the seed setting rate is low and it is difficult to obtain seedlings

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  • Tissue culture and rapid propagation method for aristolochia debilis
  • Tissue culture and rapid propagation method for aristolochia debilis
  • Tissue culture and rapid propagation method for aristolochia debilis

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] The tissue culture rapid propagation method of the mountain grass fruit, concretely carries out according to the following steps:

[0026] Step 1, the selection of explants: from April to May every year, select the young leaves of the plant that grows vigorously and has no damage by diseases and insect pests;

[0027] Step 2, sterilization of explants: the explants are washed under tap water, soaked in 0.02% potassium permanganate solution for 10 minute...

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Abstract

The invention discloses a tissue culture and rapid propagation method for aristolochia debilis. The method comprises the following specific steps: step 1, explant selection; step 2, explant sterilization; step 3, callus tissue induction culture; step 4, adventitious bud induction differentiation culture; step 5, multiplication culture; step 6, rooting culture; step 7, domestication and seedling hardening. The tissue culture and rapid propagation method disclosed by the invention can make seedling characters more stable, is suitable for standardized and scale production, is easy to generate strong seedlings and can provide high-quality and excellent seedlings with the unified standard for the market.

Description

technical field [0001] The invention belongs to the technical field of spice plant tissue culture, and relates to a tissue culture rapid propagation method of chrysanthemum fruit. Background technique [0002] Mountain grass fruit is also called Aristolochia perforatum, mountain pepper, and mountain vine grass (scientific name: Aristolochiadelavayi), which is a perennial climbing herb of the Aristolochiaceae Aristolochiaceae. It grows in limestone mountains, hills or hills at an altitude of 1600-1900 meters. In the valley bushes, the soil is barren and gravelly red soil. It is a unique wild edible spice plant in Yunnan. It has a very good effect on removing the fishy and smelly smell of meat such as cattle, sheep, fish, pigs, and dogs. It can increase the aroma and flavor of food, and also has the functions and effects of invigorating the stomach, increasing appetite, relieving lung and relieving cough. In recent years, with the development of the economy and the improvemen...

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨宝明黄玉玲李永平
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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