Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of dna dye compound with cell nucleus targeting function and application

A dye compound and cell nucleus technology, applied in organic dyes, azo dyes, luminescent materials, etc., can solve problems such as unrealizable, short wavelength of excitation light, disordered physiological processes, etc., and achieve excellent effect of cell nucleus targeting

Active Publication Date: 2020-02-18
DALIAN UNIV OF TECH
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hoechst and DAPI are two dyes that can bind to DNA, but the excitation light wavelength of Hoechst and DAPI is relatively short, and the cells will be affected by phototoxicity during the imaging process, which will cause normal physiological process disorder or cause serious cell damage, and Hoechst and DAPI can only target the nucleus but cannot achieve other functions, such as pH measurement, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of dna dye compound with cell nucleus targeting function and application
  • A kind of dna dye compound with cell nucleus targeting function and application
  • A kind of dna dye compound with cell nucleus targeting function and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025]

[0026] Under the protection of argon, compound A1 (50mg, 98.50umol) and compound B1 (55mg, 118.21umol) were added to the reaction flask, anhydrous copper sulfate aqueous solution, sodium ascorbate aqueous solution and 4 drops of N,N-diisopropyl Ethylamine was reacted at room temperature for 24h, and the solvent was evaporated under reduced pressure. The crude product was separated by column chromatography (distillate CH2Cl2:MeOH=8:1) to obtain a purple solid (60mg, 62.04umol, 62.98%). The product structure was identified by HRMS, HRMS[M+H] + : 967.4164.

Embodiment 2

[0028]

[0029] The synthetic method refers to Example 1, replaces B1 with compound B2, and the product structure is identified by HRMS, HRMS [M+H] + : 963.3864.

Embodiment 3

[0031]

[0032] The synthetic method refers to Example 1, replaces B1 with compound B3, and the product structure is identified by HRMS, HRMS [M+H] + : 1019.4374.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a DNA (deoxyribonucleic acid) dye compound with a nuclear targeting function and an application, and belongs to the field of fine chemical engineering. In the field of bioimaging, visible research of genetic materials is of great significance for cellular activity research. The wavelength of exciting light needed by dyes Hoechst and DAPI of traditional targeting cell nucleuses is short, and serious cellular damage is caused in imaging. In addition, the Hoechst and the DAPI can only purely target the cell nucleuses, but cannot achieve other functions. In order not to affect normal physiological functions of cells, the DNA dye compound has high biocompatibility, can be specifically combined with DNA and has the nuclear targeting function.

Description

technical field [0001] The invention relates to a class of DNA dye compounds with cell nucleus targeting function and applications thereof, in particular to a class of dye compounds used for cell nucleus targeting, which belongs to the field of fine chemicals. Background technique [0002] The nucleus is the main organelle for eukaryotic cells to maintain gene integrity and regulate cell functions, and contains a large amount of genetic material, namely DNA. Each eukaryotic cell contains about two meters of DNA, which interacts with proteins to form highly structured chromatin. DNA is mainly present in the nucleus in the form of chromatin or chromosomes. The regulation of cell function by the nucleus is mainly achieved through the activities of regulatory DNA. Therefore, the study of highly structured chromatin or chromosomes in the nucleus is of great significance to the study of the nucleus and the control of cell life activities. [0003] In the field of biological ima...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C09B57/00C09K11/06G01N21/64
CPCC09B57/00C09K11/06C09K2211/1059C09K2211/1088G01N21/6402
Inventor 肖义张晓东张新富
Owner DALIAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com