Method for establishing NOD-Rag2nullIL-2Rgamma null immune deficiency mouse by using gene edition technique
A technology of gene editing and defective mice, applied in other methods of inserting foreign genetic materials, genetic engineering, using microinjection, etc., to meet the research needs
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[0023] Example: The present invention uses gene editing technology to construct a method for NOD-Rag2null IL-2Rγnull immunodeficiency mice, which includes the following steps:
[0024] (1) Use CAS9 technology to knock out the Rag2 and IL-2Rγ genes in the mouse embryo genome, thereby making the mouse immune system defective, and constructing DNA that causes the immune-related genes in the mouse embryo to lose function. The sequence is as follows:
[0025] Rag2 boot sequence: GAGCGTGAGGTTGGTTGCCTG; Rag2 Donor sequences: GTCTTATAATTCTAAGACAAGCATGTAAAATGACTTTTCTTTTAAACAAGCCTTTTGTATGAGGAAGAATCTAAGCATAATTACCAATATGAAAAGATATTCTAAGTTATTGCGTTTTTTTAATCCTTTCAGATAAAAGACCTATTCACAATCAAAAATGTCCCTGCAGATGGTAACAGTGGGTCATAACATAGCCTTAATTCAACCAGGCTTCTCACTTATGAATTTTGATGGCCAAGTTTTCTTCTTTGGCCAGAAAGGCTGGCCTAAGAGATCCTGTCCTACTGGAGTCTTTCATTTTGATATAAAACAAAATCATCTCAAACTGAAGCCTGCAATCTTCTCTAAAGATTCCTGCTACCTCCCACCTCACCGTTATCCAGCTACTTGCTCATAAAAAGGCAGCATAGACTCTGACAAGCATCAATATATCATTCACGGAGGGAAAACACCAAACAATGAGCTTTCCGATAA...
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