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Preparation method and application of earthworm supernatant protein nano collagen repair compound

A technology of albumin and complex, which is applied in drug delivery, pharmaceutical formulation, medical science, etc., can solve the problems of large consumption, high economic cost, and frequent dressing changes, etc., to reduce toxicity, promote collagen repair, and enhance The effect of biocompatibility

Active Publication Date: 2019-03-15
SHAANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, protein is a biomacromolecule that is very easy to degrade in nature, which is very unfavorable for storage and transportation
In the actual medical process, there are also problems such as frequent dressing changes (large consumption) and high economic costs.

Method used

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  • Preparation method and application of earthworm supernatant protein nano collagen repair compound
  • Preparation method and application of earthworm supernatant protein nano collagen repair compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] S1. Preparation of earthworm supernatant protein: wash the fresh and alive Eisenia chinensis with 0.9% NaCl solution for several times, remove the dirt on the body surface, cut the earthworms into pieces, and use a homogenizer to crush the earthworm tissues to obtain earthworm tissue homogenate. Then add sucrose according to the ratio of 10% of the earthworm tissue homogenate quality, stir evenly, centrifuge at 4 ℃ at low speed for 10 minutes after standing still for 30 minutes, get the supernatant and be the earthworm supernatant, and get the earthworm supernatant and adopt the Bradford method ( This method is a classic textbook test method in biochemistry, so this embodiment will not repeat it, mainly for the purpose of measuring the protein concentration, so that in S3, the protein concentration can be converted to the protein amount) After measuring the protein concentration at 20 degrees Celsius, add it to the supernatant 1X anti-protein degrading enzyme to obtain e...

Embodiment 2

[0024] S1. Preparation of earthworm supernatant protein: wash the fresh and alive Eisenia chinensis with 0.9% NaCl solution for several times, remove the dirt on the body surface, cut the earthworms into pieces, and use a homogenizer to crush the earthworm tissues to obtain earthworm tissue homogenate. Then add sucrose according to the ratio of 10% of the mass of earthworm tissue homogenate, stir evenly, centrifuge at 4°C for 10 minutes at a low speed after standing still for 30 minutes, take the supernatant which is the earthworm supernatant, and use the Bradford method to obtain the earthworm supernatant After measuring the protein concentration at 20 degrees Celsius, add 1X anti-protein degrading enzyme to the supernatant to obtain an earthworm supernatant protein solution, and then add 0.02% NaN 3 As a preservative, store for later use;

[0025] S2. Preparation of nano-mesoporous transition metal carrier: Weigh 1 g of a mixture of polyvinylpyrrolidone and sodium dodecylsul...

Embodiment 3

[0028] S1. Preparation of earthworm supernatant protein: wash the fresh and alive Eisenia chinensis with 0.9% NaCl solution for several times, remove the dirt on the body surface, cut the earthworms into pieces, and use a homogenizer to crush the earthworm tissues to obtain earthworm tissue homogenate. Then add sucrose according to the ratio of 10% of the mass of earthworm tissue homogenate, stir evenly, centrifuge at 4°C for 10 minutes at a low speed after standing still for 30 minutes, take the supernatant which is the earthworm supernatant, and use the Bradford method to obtain the earthworm supernatant After measuring the protein concentration at 20 degrees Celsius, add 1X anti-protein degrading enzyme to the supernatant to obtain an earthworm supernatant protein solution, and then add 0.02% NaN 3 As a preservative, store for later use;

[0029]S2. Preparation of nano-mesoporous transition metal carrier: Weigh 1 g of a mixture of polyvinylpyrrolidone and sodium dodecylsulf...

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Abstract

The invention discloses a preparation method and application of an earthworm supernatant protein nano collagen repair compound. The preparation method comprises the following steps: preparing an earthworm supernatant protein; preparing a nano mesoporous transition metal carrier; and preparing the earthworm supernatant protein nano collagen repair compound. The earthworm supernatant protein nano collagen repair compound is applied to collagen repair of animal skin tissues. Compared to the prior art, according to the invention, an active protein ingredient is involved into a transition metal mesoporous nano material and the contact opportunity between the active ingredient and the air is reduced by PLGA, so that when the medicine effect is reserved, the earthworm supernatant protein nano collagen repair compound also has effects of reinforcing biocompatibility and reducing toxicity; and therefore, a medical pharmaceutic preparation with slow release, high efficiency, economy and biocompatibility is prepared so as to fulfill the aim of promoting the collagen repair.

Description

technical field [0001] The invention relates to the technical field of animal nano-Chinese medicine preparation, in particular to a preparation method and application of an earthworm supernatant protein nano-collagen repair compound. Background technique [0002] In daily life, various traumatic events occur from time to time. With the development of society and economy, the number of trauma cases caused by traffic accidents, medical trauma and other reasons is also increasing year by year. When trauma occurs, organisms will immediately start their own defect repair mechanism. Wound repair is generally considered to be divided into three distinct phases: the inflammatory phase, the proliferative phase, and the maturation phase. From the inflammatory phase, injured tissue can begin to secrete collagen for collagen deposition. After about three days, wound repair enters the proliferative phase, when the tissue secretes type III and type V collagen. At this time, the collag...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/22A61L27/50A61L27/18A61L27/56A61L27/04A61L27/54
CPCA61L27/042A61L27/045A61L27/047A61L27/18A61L27/227A61L27/50A61L27/54A61L27/56A61L27/60A61L2300/252A61L2300/412A61L2300/602A61L2400/12C08L89/00C08L67/04
Inventor 王东谷婷刘韦王瑞辉张红
Owner SHAANXI UNIV OF CHINESE MEDICINE
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