Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A solid nanoporous structure

A nanopore structure and nanopore array technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of reducing the detection accuracy of tumor markers, and achieve the effect of simple structure and improved detection accuracy

Active Publication Date: 2020-07-03
GUANGDONG UNIV OF TECH
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, the tumor markers captured by the antibody at the edge of the orifice are not blocked from the expression of the current signal, thereby reducing the detection accuracy of the tumor markers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A solid nanoporous structure
  • A solid nanoporous structure
  • A solid nanoporous structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Such as figure 1 As shown, the present embodiment provides a solid-state nanopore structure, which comprises a sandwich structure nanohole array 1, and the sandwich structure nanohole array 1 is composed of a top layer 10, a middle layer 11 and a bottom layer 12 arranged in sequence , the top layer 10 and the bottom layer 12 are protective layer nanohole arrays, the material of the protective layer nanopore arrays is silicon oxide, the middle layer 11 is a biomolecular modification position layer, and the material of the biomolecular modification position layer is silicon, and the biomolecular for the antibody.

[0024] Such as figure 2 and image 3 As shown, when performing surface chemical modification, the nanopore array materials of the top layer 10 and the bottom layer 12 do not react, or the surface groups generated by the reaction cannot be stably combined with the functional groups of biomolecules, and the surface groups generated by the reaction of the mater...

Embodiment 2

[0029] The present embodiment provides a solid nanopore structure, which comprises a sandwich structure nanohole array 1, the sandwich structure nanohole array 1 is composed of a top layer 10, a middle layer 11 and a bottom layer 12 arranged in sequence, the described The top layer 10 and the bottom layer 12 are nanohole arrays in the protective layer, and the material of the nanohole array in the protective layer is silicon nitride. Needle DNA.

[0030] When performing surface chemical modification, the nanopore array materials of the top layer 10 and the bottom layer 12 do not react, or the surface groups generated by the reaction cannot be stably combined with the biomolecular functional groups, and the surface groups generated by the reaction of the material of the middle layer 11 are stable with the biomolecular functional groups Combination, the biomolecule is the probe DNA, the biomolecule described in this embodiment is the probe DNA2 paired with the methylated DNA3, w...

Embodiment 3

[0035] The present embodiment provides a solid nanopore structure, which comprises a sandwich structure nanohole array 1, the sandwich structure nanohole array 1 is composed of a top layer 10, a middle layer 11 and a bottom layer 12 arranged in sequence, the described The top layer 10 and the bottom layer 12 are nanohole arrays in the protective layer, and the material of the nanohole arrays in the protective layer is aluminum oxide. Needle.

[0036]When performing surface chemical modification, the nanopore array materials of the top layer 10 and the bottom layer 12 do not react, or the surface groups generated by the reaction cannot be stably combined with the biomolecular functional groups, and the surface groups generated by the reaction of the material of the middle layer 11 are stable with the biomolecular functional groups Combination, the biomolecule is the probe RNA. The biomolecule described in this example is the probe RNA2 paired with microRNA3. When performing sur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a novel solid-state nano-pore structure, and relates to the technical field of micro-nano medical detection application. The novel solid-state nano-pore structure is applied toaccurately controlling a modification position of a biomolecule. The solid-state nano-pore structure includes a sandwich structure nano-pore array; the nano-pore array consists of a top layer, a middle layer and a bottom layer arranged sequentially; each of the top layer and the bottom layer is a protective layer of the nano-pore array; and the middle layer is a biomolecule modification positionlayer. The novel solid-state nano-pore structure is simple in structure; by using a difference of a binding force between a material in a nano-pore sandwich structure and a functional group of the biomolecule, the accurate control on the modulation position of the biomolecule is implemented, thus being beneficial to improving the detection accuracy of the biomolecule; and the novel solid-state nano-pore structure has a wide use prospect in the field of accurate medical detection.

Description

technical field [0001] The invention relates to the technical field of micro-nano medical detection applications, in particular to a solid-state nanopore structure. Background technique [0002] In 1996, Kasianowicz et al. (Kasianowicz J J, Brandin E, Branton D, et al. Characterization of individual polynucleotide molecules using a membrane channel [J]. Proceedings of the National Academy of Sciences, 1996, 93(24): 13770-13773.) A nanopore sequencing method is proposed. When the negatively charged DNA molecules are driven through the nanopore by an electric field, the physical occupation of the base generates a blocking current signal, and the sequencing is realized by measuring the amplitude and time characteristics of the signal. Nanopore sequencing inspires biomolecular detection research. Based on the blocking current theory of nanopore sequencing, single nanopore sensors can identify single biomolecules, such as tumor marker molecules: methylated DNA, microRNA, etc. Q...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B81B1/00B81B7/04G01N33/543B82Y5/00B82Y30/00B82Y40/00
CPCB81B1/00B81B7/04B82Y5/00B82Y30/00B82Y40/00G01N33/54306
Inventor 袁志山谢志鹏王成勇
Owner GUANGDONG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com