Combined inactivate vaccine for sheep pox and contagious caprine pleuropneumonia (CCPP), and production method of combined inactivate vaccine

Abstract

The invention relates to a combined inactivate vaccine for sheep pox and contagious caprine pleuropneumonia (CCPP), and a production method of the combined inactivate vaccine. The method comprises thesteps of inoculating suspension cultured BHK-21 cells and a proper culture medium with a capripox virus AV41 strain and a mycoplasma capricolum pneumonia subspecies C87001 strain respectively for culturing, harvesting the culture, concentrating and purifying; inactivating with BEI and thimerosal respectively, adding a suitable adjuvant according to a certain proportion, mixing and emulsifying. The combined inactivate vaccine is used for preventing the sheep pox and the CCPP. The combined inactivate vaccine, provided by the invention, for the sheep pox and the CCPP is obtained internationallyfor the first time, and can achieve the aim of preventing the two diseases by means of one injection; furthermore, a live vaccine for the sheep pox is produced by means of the suspension cultured BHK-21 cells; compared with the original inactivate vaccine prepared from ovine testicular cells, the combined inactivate vaccine provided by the invention has obvious advantages, and can solve the problems that in the production process of the live vaccine for the sheep pox, the sheet testis is difficult to obtain, lamb death and ewe abortion are easily caused, subcutaneous immunization needs to be performed when the vaccine is used, an operation is difficult during clinical application, and the like.

Description

technical field [0001] The invention relates to a dual inactivated vaccine of sheep pox and goat infectious pleuropneumonia and a production method thereof. It belongs to the field of veterinary biological products. Background technique [0002] The sheep raising industry is closely related to the development of the national economy and the improvement of the living standards of the people of all ethnic groups. Especially after entering the 21st century, the sheep raising industry in my country has developed rapidly with remarkable results, and the proportion of the sheep raising industry in the national economy has also increased year by year. . According to data from the Food and Agriculture Organization of the United Nations (FAO), in 2013, the number of goats in mainland China reached 183 million. In 2014, the number of goats in mainland China was 188 million. Although the number of sheep raised in my country ranks first in the world, compared with countries with devel...

AI Technical Summary

Problems solved by technology

Among them, the goat pox live vaccine widely used in my country has been tested by long-term clinical practice and found to have the following three major deficiencies. Larger potential safety hazards; second, it is necessary to use primary sheep testicular cells to prepare vaccines, and the source of sheep testes is difficult in the production process, and there is a biosafety risk of primary cells carrying exogenous viruses; third, intradermal immunization is required, Difficult to handle during clinical use

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