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Method for extracting flos abelmoschus manihot extract

A technology for extraction of hollyhock flowers and extracts, which is applied in the field of extraction of hollyhock flower extracts, achieving the effect of short time and high content

Active Publication Date: 2019-03-08
JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no efficient method for simultaneously extracting quercetin, isoquercetin and hyperoside

Method used

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  • Method for extracting flos abelmoschus manihot extract
  • Method for extracting flos abelmoschus manihot extract
  • Method for extracting flos abelmoschus manihot extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The contrast 1 of embodiment 1 reflux solvent

[0026] Get two portions of hollyhock flowers (purchased from Taizhou Gaogang Chinese Herbal Pieces Co., Ltd.), each portion of 10g, respectively into 250ml flasks. Add 100ml of 70% methanol and 70% ethanol respectively, adjust the temperature of the water bath to 80°C, reflux for 0.5 hours, take back the effluent and filter it, inject it into a high-performance liquid chromatograph, and measure quercetin and isoquercetin in it. The content of element and hyperoside. The measured results are shown in Table 1.

[0027] Table 1 Determination of reflux solvent

[0028] extraction conditions

[0029] Conclusion: The yield of extracts extracted from hollyhock flowers by using 70% methanol is higher than that extracted by 70% ethanol.

Embodiment 2

[0030] The contrast 2 of embodiment 2 reflux solvent

[0031] Get 7 parts of 10g hollyhock flowers respectively, and put them in 250ml flasks respectively. Add 100ml of methanol with a concentration of 100%, 90% methanol, 80% methanol, 70% methanol, 60% methanol, 50% methanol, and 40% methanol, and adjust the temperature of the water bath to 80°C and reflux at 0.5 Hours, take back the fluid and filter it, inject it into a high-performance liquid chromatograph, and measure the contents of quercetin, isoquercetin and hyperin in it. The measured results are shown in Table 2.

[0032] Table 2 Determination of reflux solvent concentration

[0033]

[0034]

[0035] Conclusion: This example can prove that the yield of the extract of the hollyhock flower extracted by using the solution with a concentration of 50-80% methanol is higher, and the yield of the extract of the hollyhock flower extracted with the concentration of 70% methanol is the highest.

Embodiment 3

[0036] The influence of embodiment 3pH

[0037] Get 5 parts of 10g of hollyhock flowers respectively, and put them into 250ml flasks respectively. Add 100ml of methanol aqueous solution with acetic acid to adjust the pH value to be respectively, pH=4.0, pH=4.5, pH=4.8, pH=5.0, pH=5.5, wherein the concentration of methanol is 70%, adjust the temperature of the water bath to 80°C, and reflux After 0.5 hour, take back the effluent and filter it, inject it into a high-performance liquid chromatograph, and measure the content of quercetin therein. The measured results are shown in Table 3.

[0038] The influence of table 3 extraction solvent PH

[0039] extraction conditions

[0040] Conclusion: This example can prove that the active ingredient extracted by using the 70% methanol solution with a pH of 4.5-5.0 is higher, and the active ingredient extracted by the 70% methanol solution with a pH of 4.8 is the highest.

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Abstract

The invention provides a method for extracting flos abelmoschus manihot extract. The method comprises the following steps: heating for refluxing and extracting flos abelmoschus manihot for 2 to 3 times by adopting a methanol solution with a concentration of 50 to 80 percent, so as to obtain an extracting solution, wherein each time is lasted for 0.5 to 1 h; decompressing concentrating the extracting solution under a condition that the temperature is lower than 60 DEG C to obtain extract with a proportion of 1.13 to 1.16 g / ml; adding water which is 2 to 4 times the volume of the extract; centrifuging for precipitating, and taking supernatant; taking the centrifuged and precipitated supernatant to pass through a macroporous adsorbent resin column; after eluting for 2 to 3 column volumes by adopting water, then eluting for 3 column volumes by adopting a methanol solution with a concentration of 20 percent; eluting for 1 column volume by adopting a methanol solution with a concentration of50 to 70 percent; concentrating an eluting solution; spray-drying to obtain the extract. The content of total flavonoids in the flos abelmoschus manihot extract obtained by the method provided by theinvention reaches 70 to 90 percent according to a weight percent; meanwhile, effective components including hyperoside, quercetin and isoquercetin can be extracted at the same time; the time is shortand the content is high.

Description

technical field [0001] The invention belongs to the field of pharmacy, and in particular relates to a method for extracting extracts from hollyhock flowers. Background technique [0002] The flower of hollyhock (Abelmoschlmanihot L medic) is the dried flower of hollyhock, a plant of Malvaceae. There are longitudinal veins on the surface, radial, light brown, shallow wavy edges, more shrunken and broken, and the complete petals are triangular, broad and obovate, with longitudinal veins on the surface, radial, light brown, shallow wavy edges; the inner base is purple-brown . Stamens numerous, united into tubular, anthers subsessile. Stigma purple-black, spoon-shaped, 5-lobed. The gas is slightly fragrant, and the taste is sweet and light. [0003] It is sweet and cold in nature, non-toxic, and has the effects of clearing away dampness and heat, reducing inflammation and detoxification. It can be taken orally to treat stranguria and edema; it can be used externally to treat...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K133/00
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 段琼辉李永秦枫胡新岗陈玉勇祁兴普陈巍
Owner JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE
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