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Method for preparing cells

A cell and equipment technology, applied in the field of cell preparation, can solve problems such as single function and complex structure

Inactive Publication Date: 2019-03-01
EUREKA BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned existing technologies can only complete part of the steps of cell preparation or can only be used for the steps of cell separation, and have single functions and complex structures, which need further optimization.

Method used

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  • Method for preparing cells
  • Method for preparing cells
  • Method for preparing cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0135] Embodiment 1 equipment assembly

[0136] This embodiment provides a device, which includes a sampling device, a collection device, a liquid replacement device, a centrifugal culture device, a liquid sensor device, a gas exchange device, a weighing component device, and a magnet control device;

[0137] The liquid sensor device includes a first liquid sensor and a second liquid sensor, wherein the first liquid sensor is connected to the sampling device, and the second liquid sensor is connected to the centrifugal culture device; the weighing component device includes a load cell, respectively Set on the sampling device, collection device, centrifugal culture device and fluid replacement device; wherein, the sampling device, centrifugal culture device, gas exchange device, magnet control device, fluid replacement device and collection device are connected in sequence; the sampling device It includes a blood bag connector 1, a blood clot filter 31 and a first tube clamp 8;...

Embodiment 2

[0139] Embodiment 2 prepares cells

[0140] Cells were prepared as follows:

[0141] (1) Prepare a 100mL blood sample, connect consumables, and perform a self-test. The consumables include a blood sample bag 35, a lymphatic separation fluid bag 5, a first collection bag 6, a second collection bag 7, physiological saline bags 33-34, culture Base bag 4 and centrifuge bucket 29;

[0142] The gradient centrifugation method is used to carry out centrifugation in the centrifugal culture device, and the liquid sensor device, the centrifugal culture device and the weighing component device cooperate to adjust the liquid flow, and the cells in the sample are separated to the collection device;

[0143] Draw the physiological saline in the liquid replacement device into the centrifuge bucket 29, then drain it into the lymphatic separation liquid bag 5, and repeat it several times;

[0144] (2) Add antibody magnetic beads to the cells collected in step (1), transfer to a centrifugal cu...

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Abstract

The invention provides a method for preparing cells. The method adopts equipment for preparing the cells; the equipment is obtained by modifying on the basis of a full-automatic blood component separation machine and is used for culturing and preparing the cells; a weighing sensor is used for replacing an optical grating, and an air inlet device and a liquid sensor are additionally arranged; a connection relation of all devices in the whole equipment is optimized and an operation flow of the equipment is simplified; all steps of all the devices are mutually matched, the production cost is reduced and a technological flow is integrated, so that a cell preparation process is more intelligent and automatic and the operation is more convenient; the pollution to the cells is reduced and the success rate of preparing the cells is improved; the method has a wide application prospect and great market value.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a method for preparing cells. Background technique [0002] Cell therapy is a method of separating and extracting some of the cells from human or animal tissues and blood, and directly infusing them back into humans or animals, or by screening, genetically modifying, inducing differentiation, and culturing and expanding some of the extracted cells. A form of treatment that is infused back into humans or animals. Cellular immunotherapy is a rapidly developed cell therapy for various diseases in recent years. It achieves the purpose of curing diseases by reinfusing in vitro screened, modified, induced, and expanded lymphocytes in tissues or blood; common applications include preparation of dendrites Stem cells (DC), T cells, NK cells to treat cancer; prepare Treg cells to treat autoimmune diseases and treat various diseases such as infertility, allergies, and viral infections. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/36C12M1/34C12M1/12C12M1/04C12N5/078
CPCC12M23/14C12M29/04C12M33/10C12M33/14C12M37/04C12M41/22C12M41/32C12M41/40C12M47/04C12N5/0634C12N2509/00C12M45/05
Inventor 马墨薛博夫陈林雄郑伟武
Owner EUREKA BIOTECH LTD
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