Automatic nucleic acid extracting device applicable to space

Abstract

The invention discloses an automatic nucleic acid extracting device applicable to space. The device comprises a translation table, a stepping motor, a limiting switch, an injector set, a silicon rubber tube, a pressing pipe valve, electromagnets and a control circuit. The injector set is formed by fixedly connecting five 1 ml injectors for storing reagents needed by nucleic acid extraction; the injectors are fixed to the translation table, the translation table is driven by the stepping motor and can push and pull pistons of the injectors; the limiting switch is located at the end of the stroke of the pistons of the injectors and used for feeding back the position of the motor; the injectors are connected together through the silicon rubber tube, and the pressing pipe valve is used for controlling on-off of the silicon rubber tube; the two electromagnets are located at the two sides of the main injector for generating an oscillation magnetic field. The full-automatic extracting processfrom samples to purified nucleic acid inside a liquid closed system is achieved, no risk of aerosol contaminations is caused, the device can be used for automatically extracting nucleic acid of biological samples under the micro-gravity environment, and integration with a follow-up nucleic acid processing module is facilitated.

Description

technical field [0001] The invention relates to space biotechnology, in particular to a device suitable for the microgravity environment of space and capable of automatically extracting nucleic acids. Background technique [0002] Nucleic acid is an important object of modern molecular biology detection. The primary problem of nucleic acid detection is to extract the required nucleic acid from biological samples. The nucleic acid extraction methods commonly used in the laboratory include manual extraction and automatic extractor extraction. Manual extraction requires sample cracking, adsorption, cleaning, and elution in sequence. The steps required are cumbersome, and the liquid is easily volatile in the air, and the sample is easily contaminated . The laboratory nucleic acid automatic extractor uses a robotic arm to automatically implement the manual extraction steps in the machine. The test tubes used are also open, and there is also the risk of sample contamination. The...

AI Technical Summary

Problems solved by technology

However, the nucleic acid extraction instruments commonly used in ground laboratories use the gravity environment to keep the liquid at the bottom of the test tube, and operate the liquid in the microgravity of space. There is no fixed gas-liquid interface, and the ground device cannot be used directly in space.

At the same time, complex operations in the space station have high requirements for experimental operators. Therefore, my country's space station needs to study automatic nucleic acid extraction technology suitable for space applications

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