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Simplicillium pekinense strain LYZ7 and application thereof

A technology of Paecilomyces and strains, applied in the field of microorganisms, can solve the problems of limited degradation effect and insufficient degradation of tetracycline, and achieve significant degradation effect and good application prospects

Active Publication Date: 2019-02-15
BEIJING DONGXIANG ENVIRONMENTAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, it is far from enough to rely on these strains to degrade tetracycline. On the one hand, the degradation effect is limited, and on the other hand, the selection is also limited. Therefore, it is urgent to develop more new strains in this field, and even find new strains for effective degradation. tetracycline

Method used

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  • Simplicillium pekinense strain LYZ7 and application thereof
  • Simplicillium pekinense strain LYZ7 and application thereof
  • Simplicillium pekinense strain LYZ7 and application thereof

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experiment example

[0065] Experimental example, screening and verification process of the strain of the invention

[0066] 1Materials and methods

[0067] 1.1 Material

[0068] 1.1.1 Source of strain

[0069] The soil samples used in the experiment were taken from the humus soil under the woods of Beijing Agricultural College.

[0070] 1.1.2 Medium

[0071] Basic inorganic salt medium: CaCl 2 , 0.02g; (NH 4 ) 2 SO 4 , 1.0g; NaCl, 0.5g; FeCl 3 , 0.05g; K 2 HPO 4 , 1.0g; NH 4 NO 3 , 1.0g; KH 2 PO 4 , 1.0g; pH=7.0;

[0072] Trace element solution: CoCl 2 ·6H 2 O, 2g; MnCl 2 ·4H 2 O, 8.5g; ZnCl 2 , 0.1g; NiCl 2 ·6H 2 O, 0.2g; CuSO 4 ·5H 2 O, 0.3g; Na 2 MoO 4 ·2H 2 O, 0.2g; Na 2 SeO 4 ·2H 2 O, 0.2g dilute to a 1L volumetric flask for later use;

[0073] Screening medium: add tetracycline to sterile inorganic salt medium and configure it to different concentrations according to the needs of the experiment; the addition amount of trace elements is 1mL·L -1 ;

[0074] PDA medium: 200g peeled potatoes (add distilled w...

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Abstract

The invention discloses a Simplicillium pekinense strain LYZ7 and application thereof, and belongs to the field of microorganisms. The preservation number of the strain LYZ7 is CGMCC No.16487. The invention accidentally finds that the strain belongs to a novel strain and is named as the Simplicillium pekinense. Based on the strain, the invention also provides an inoculant which can degrade tetracycline, a tetracycline biodegradable agent and a tetracycline biodegradation method. The strain LYZ7 disclosed by the invention has an obvious degradation effect on the tetracycline; the degradation rate reach as high as 89.21 percent after the tetracycline is treated for 14 days.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a strain of Paecilomyces beijingensis strain LYZ7 and its application. Background technique [0002] In recent years, with the development of my country's aquaculture industry, tetracycline antibiotics have been widely used due to their low price and remarkable effects. Due to the low absorption rate of such antibiotics in animals, most of them will enter the environment with animal excrement and accumulate in the environment, posing a threat to environmental safety and human health. [0003] There are many ways to deal with antibiotic pollution, mainly divided into non-biodegradation and biodegradation. Abiotic degradation is mainly to degrade antibiotics through physical or chemical means, such as photolysis, hydrolysis and oxidative degradation. Although non-biodegradation can quickly remove antibiotic residues, the chemical materials used for antibiotic degradati...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F11/02C02F3/34B09C1/10C12R1/645C02F103/20
CPCB09C1/105C02F3/34C02F11/02C02F2103/20C12N1/145C12R2001/645
Inventor 刘悦秋尹静冯佳宁
Owner BEIJING DONGXIANG ENVIRONMENTAL TECH CO LTD
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